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MB Sample ID: SA333320
| Local Sample ID: | P220801163 |
| Subject ID: | SU003210 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003210 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| P220801163 | SA333320 | FL039588 | serum | Sample source |
| P220801163 | SA333320 | FL039588 | intervention | Factor |
Collection:
| Collection ID: | CO003203 |
| Collection Summary: | Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003219 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003216 |
| Sampleprep Summary: | Samples were added in a 2 mL centrifuge tube, add 10 μL of internal standard solution, then add 750 μL of 1% formic acid-acetonitrile solution, vortex, centrifuge for 10 min at 12,000 rpm at 4^oC, and then resovle and filter the supernatant by 0.22 μm membrane for LC-MS analysis. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005066 |
|---|---|
| Analysis type | MS |
| Chromatography type | LC |
| Chromatography system | AB SCIEX Jasper HPLC |
| Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE |
| Units | μmol/L |
Chromatography:
| Chromatography ID: | CH003825 |
| Instrument Name: | AB SCIEX Jasper HPLC |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~1 min, 80% B; 1~2 min, 80~70% B; 2~2.5 min, 70% B; 2.5~3 min, 70~50% B; 3~3.5 min, 50% B; 3.5~4 min, 50~80% B; 4~6 min, 80% B |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | LC |
MS:
| MS ID: | MS004804 |
| Analysis ID: | AN005066 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Ion Source Temperature 500°C, Ion Source Voltage 5500 V, Collision Gas 6 psi, Curtain Gas 30 psi, Atomizing Gas and Auxiliary Gas both 50 psi. Multiple reaction monitoring (MRM) was employed. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | POSITIVE |
