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MB Sample ID: SA333348
| Local Sample ID: | 180MN |
| Subject ID: | SU003213 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003213 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 180MN | SA333348 | FL039594 | cecum content | Sample source |
| 180MN | SA333348 | FL039594 | intervention | Factor |
Collection:
| Collection ID: | CO003206 |
| Collection Summary: | Cecum content was collected and stored at -80°C environment for tryptophan analysis. |
| Sample Type: | Cecum |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003222 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003219 |
| Sampleprep Summary: | Sample was extracted in 100 μL of 80% methanol aqueous solution, high throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol aqueous solution, high throughput tissue grinder grinding for 120 s. Centrifugation was carried out at 12,000 rpm at 4 ℃ for 10 min, and the supernatant was filtered through the membrane to add an equal amount of the internal standard solution, and then transferred into the vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005069 |
|---|---|
| Analysis type | MS |
| Chromatography type | LC |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5000 |
| Ion Mode | POSITIVE |
| Units | μg/g |
Chromatography:
| Chromatography ID: | CH003828 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~2 min, 1% B; 2~3 min, 1~30% B; 3~3.5 min, 30% B; 4.5~8 min, 30~50% B; 8~10 min, 50~95% B; 10~11 min, 95% B; 11~17 min, 95~1% B. |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in methanol |
| Chromatography Type: | LC |
MS:
| MS ID: | MS004807 |
| Analysis ID: | AN005069 |
| Instrument Name: | ABI Sciex 5000 |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Ion Source Temperature 500°C, Ion Source Voltage 5500 V, Collision Gas 6 psi, Curtain Gas 30 psi, Atomizing Gas and Auxiliary Gas both 50 psi. 45 arbitrary units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep gas, ion transfer tube, and vaporizer temperature, respectively. The ion source was operated using heated ESI with an ion spray voltage set at 5500 V in positive ion mode. Polarity switching and scheduled selected reaction monitoring (SRM) were employed.The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | POSITIVE |
