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MB Sample ID: SA333351
| Local Sample ID: | 188S14 |
| Subject ID: | SU003214 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003214 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 188S14 | SA333351 | FL039595 | fecal | Sample source |
| 188S14 | SA333351 | FL039595 | control | Factor |
Collection:
| Collection ID: | CO003207 |
| Collection Summary: | Feces were collected at and stored at -80°C for SCFAs analysis. |
| Sample Type: | Feces |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003223 |
| Treatment Summary: | mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003220 |
| Sampleprep Summary: | 100 mg of sample was extracted in 50 μL of 15% phosphoric acid with 100 μL of 125 μg/mL 4-methyl valeric acid solution as internal standard and 400 μL ether, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for 1 min and the supernatant was transferred into the vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005070 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo Trace 1310 |
| Column | Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo ISQ LT |
| Ion Mode | UNSPECIFIED |
| Units | μg/g |
Chromatography:
| Chromatography ID: | CH003829 |
| Instrument Name: | Thermo Trace 1310 |
| Column Name: | Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm) |
| Column Temperature: | The column temperature was programmed to increase from an initial temperature of 90°C, followed by an increase to 120 °C at 10 °C/min, and to 150 °C at 5 °C/min, and finally to 250 °C at 25 °C/min which was maintained for 2 min |
| Flow Gradient: | none |
| Flow Rate: | 1 mL/min |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | GC |
MS:
| MS ID: | MS004808 |
| Analysis ID: | AN005070 |
| Instrument Name: | Thermo ISQ LT |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Single ion monitoring (SIM) mode was used with the electron energy of 70 eV.MS Convert software (Proteowizard, v3.0.8789) was applied to convert “.raw” format raw data to “.mzML” format, which would be used for downstream analysis. |
| Ion Mode: | UNSPECIFIED |
