Return to study ST003105 main page
MB Sample ID: SA333645
| Local Sample ID: | 6 |
| Subject ID: | SU003220 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003220 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 6 | SA333645 | FL039615 | Plasma | Sample source |
| 6 | SA333645 | FL039615 | Wild-type | Genotype |
Collection:
| Collection ID: | CO003213 |
| Collection Summary: | Blood was collected in EDTA-coated tubes, and plasma was obtained through centrifugation (7000 x g, 7 min, 4°C), snap frozen in liquid nitrogen and stored at -80°C until further use. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR003229 |
| Treatment Summary: | Control mice (wild-type) and mice expressing hM3Dq in GFRAL neurons (transgenic) were given CNO (1 mg/kg) i.p. and bled 6 hours later for NMR metabolomics. |
Sample Preparation:
| Sampleprep ID: | SP003226 |
| Sampleprep Summary: | Plasma was thawed at room temperature for 15 min before transferring 150 µl to pre-washed ultrafiltration tubes (Amicon Ultra 0.5 ml, 3k MWCO, Merck Millipore) which were spun at 14000 x g, at 4°C for 90 min. 90 µl of each filtrate was mixed with 90 µl buffer (75 mM sodium phosphate pH 7.4, 0.1% w/v sodium azide, 3.66 mM 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid (TSPd4), in 20% v/v D2O) and transferred to 3 mm SampleJet NMR-tubes. |
Analysis:
| MB Sample ID: | SA333645 |
| Analysis ID: | AN005084 |
| Analysis Type: | NMR |
| Num Factors: | 2 |
| Num Metabolites: | 49 |
| Units: | mM |
NMR:
| NMR ID: | NM000276 |
| Analysis ID: | AN005084 |
| Instrument Name: | Bruker Neo |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 599.75 MHz |
