Return to study ST003127 main page

MB Sample ID: SA338953

Local Sample ID:HL_WTCD_8
Subject ID:SU003244
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU003244
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
HL_WTCD_8SA338953FL040136HeartSample source
HL_WTCD_8SA338953FL040136WTGenotype
HL_WTCD_8SA338953FL040136Control DietTreatment
HL_WTCD_8SA338953FL040136MSex
HL_WTCD_8SA338953FL04013675Age

Collection:

Collection ID:CO003237
Collection Summary:Murine heart tissue was excised and snap frozen in liquid N2.
Sample Type:Heart

Treatment:

Treatment ID:TR003253
Treatment Summary:CHCHD10 WT and CHCHD10 S55L heterozygous mice were treated with either a control diet (70% Carbohydrate, 20% Protein, 10% Fat) or High Fat Diet (60% Fat, 20% Protein, 20% Carbohydrate) in utero until 75 days of age.

Sample Preparation:

Sampleprep ID:SP003251
Sampleprep Summary:In brief, 1 ml of 90% isopropanol was added to 15 mg of homogenized heart tissue. Samples were vortexed for 2 min, sonicated for 1 min using a probe sonicator and centrifuged at 15,000 g for 10 min. The supernatant was collected and dried down using a SpeedVac. The dried samples were reconstituted using acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS analysis.

Combined analysis:

Analysis ID AN005126 AN005127
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um) Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Intensity Peak Intensity

Chromatography:

Chromatography ID:CH003879
Methods Filename:Method_Lipidomics.pdf
Instrument Name:Thermo Vanquish
Column Name:Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um)
Column Temperature:35
Flow Gradient:0–1.5 min, 32% B; 1.5-4 min, 32-45% B; 4-5 min, 45-52% B; 5-8 min, 52-58% B; 8-11 min, 58-66% B; 11-14 min, 66-70% B; 14-18 min, 70-75% B; 21-25 min, isocratic 97% B, 25-25.1 min 97-32% B
Flow Rate:200 µl/min
Solvent A:60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B:90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004862
Analysis ID:AN005126
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:A data-dependent mass spectrometric acquisition method was used for lipid identification. In this method, each MS survey scan was followed by up to 10 MS/MS scans performed on the most abundant ions. Data was acquired in positive and negative mode in separate runs. The following electrospray parameters were used: spray voltage 3.0 kV, heated capillary temperature 350 °C, HESI probe temperature 350 °C, sheath gas, 35 units; auxiliary gas 10 units. For MS scans: resolution, 70,000 (at m/z 200); automatic gain control target, 3e6; maximum injection time, 200 ms; scan range, 250-1800 m/z. For MS/MS scans: resolution, 17,500 (at 200 m/z); automatic gain control target, 1e5 ions; maximum injection time, 75 ms; isolation window, 1 m/z; NCE, stepped 20,30 and 40. The LC-MS results were processed using MS-DIAL software (version 4.9) for lipid identification and relative quantitation.
Ion Mode:POSITIVE
Analysis Protocol File:Method_Lipidomics.pdf
  
MS ID:MS004863
Analysis ID:AN005127
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:A data-dependent mass spectrometric acquisition method was used for lipid identification. In this method, each MS survey scan was followed by up to 10 MS/MS scans performed on the most abundant ions. Data was acquired in positive and negative mode in separate runs. The following electrospray parameters were used: spray voltage 3.0 kV, heated capillary temperature 350 °C, HESI probe temperature 350 °C, sheath gas, 35 units; auxiliary gas 10 units. For MS scans: resolution, 70,000 (at m/z 200); automatic gain control target, 3e6; maximum injection time, 200 ms; scan range, 250-1800 m/z. For MS/MS scans: resolution, 17,500 (at 200 m/z); automatic gain control target, 1e5 ions; maximum injection time, 75 ms; isolation window, 1 m/z; NCE, stepped 20,30 and 40. The LC-MS results were processed using MS-DIAL software (version 4.9) for lipid identification and relative quantitation.
Ion Mode:NEGATIVE
Analysis Protocol File:Method_Lipidomics.pdf
  logo