Summary of Study ST000372

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000372
Study TitleMeasure change in metabolites based on diet and feeding status (part I)
Study TypeRegular
Study SummaryFlies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-18
Num Groups4
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HP4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000134
Project DOI:doi: 10.21228/M8HP4Z
Project Title:Fly Brain Studies
Project Summary:Measure change in metabolites according to diet and feeding state
Institute:University of Michigan
Department:MCDB LS&A
Laboratory:Dus Lab
Last Name:Dus
First Name:Monica
Address:Ann Arbor, MI
Email:mdus@umich.edu
Phone:347-891-2287

Subject:

Subject ID:SU000393
Subject Type:Animal
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Species Group:Insect

Factors:

Subject type: Animal; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id State
SA016950S00018443sated | Diet:high sugar
SA016951S00018447sated | Diet:high sugar
SA016952S00018446sated | Diet:high sugar
SA016953S00018444sated | Diet:high sugar
SA016954S00018445sated | Diet:high sugar
SA016955S00018451sated | Diet:normal
SA016956S00018449sated | Diet:normal
SA016957S00018450sated | Diet:normal
SA016958S00018448sated | Diet:normal
SA016959S00018458starved | Diet:high sugar
SA016960S00018459starved | Diet:high sugar
SA016961S00018460starved | Diet:high sugar
SA016962S00018457starved | Diet:high sugar
SA016963S00018452starved | Diet:normal
SA016964S00018453starved | Diet:normal
SA016965S00018454starved | Diet:normal
SA016966S00018455starved | Diet:normal
SA016967S00018456starved | Diet:normal
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000387
Collection Summary:none
Sample Type:Tissues

Treatment:

Treatment ID:TR000407
Treatment Summary:none
Treatment Protocol Filename:Acyl-CoA_-20150305.docx

Sample Preparation:

Sampleprep ID:SP000400
Sampleprep Summary:none
Sampleprep Protocol Filename:Acyl-CoA_-20150305.docx

Combined analysis:

Analysis ID AN000606
Analysis type MS
Chromatography type Reversed phase
Chromatography system None
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410 QQQ
Ion Mode POSITIVE
Units pmol/µg tissue protein

Chromatography:

Chromatography ID:CH000432
Methods ID:DQM0127
Methods Filename:QM-005-Xbrdg2mm-CoA-6490.m.zip
Instrument Name:None
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000539
Analysis ID:AN000606
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:QM-005-Xbrdg2mm-CoA-6490.m.zip
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