Summary of Study ST001327
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000905. The data can be accessed directly via it's Project DOI: 10.21228/M8Q69W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001327 |
| Study Title | Mitochondrial Lipid Profiles in Traumatic Optic Neuropathy |
| Study Summary | C57Bl/6J Mice were exposed to sonication to induce a traumatic optic neuropathy model. Optic nerves were harvested and mitochondrial isolation was performed. Lipids were then extracted from isolated mitochondria. Mass spectrometry was used to analyze mitochondrial lipids. |
| Institute | University of Miami |
| Department | Ophthalmology, Bascom Palmer Eye Institute |
| Last Name | Bhattacharya |
| First Name | Sanjoy K |
| Address | 1638 NW 10th Avenue, Suite 707A University of Miami Miami, FL, 33136 |
| SBhattacharya@med.miami.edu | |
| Phone | 305-482-4103 |
| Submit Date | 2020-03-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-03-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000905 |
| Project DOI: | doi: 10.21228/M8Q69W |
| Project Title: | Mitochondrial Lipid Profiles in Traumatic Optic Neuropathy |
| Project Summary: | C57Bl/6J Mice were exposed to sonication to induce a traumatic optic neuropathy model. Optic nerves were harvested and mitochondrial isolation was performed. Lipids were then extracted from isolated mitochondria. Mass spectrometry was used to analyze mitochondrial lipids. |
| Institute: | University of Miami |
| Department: | Bascom Palmer Eye Institute |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy K |
| Address: | 1638 NW 10th Avenue, Suite 707A University of Miami Miami, FL, 33136 |
| Email: | SBhattacharya@med.miami.edu |
| Phone: | 305-482-4103 |
Subject:
| Subject ID: | SU001401 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 2 Months |
| Gender: | Male and female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Exposure |
|---|---|---|
| SA096333 | C7 | No Exposure | Days Post Exposure:Control | Sex:F |
| SA096334 | C8 | No Exposure | Days Post Exposure:Control | Sex:F |
| SA096335 | C6 | No Exposure | Days Post Exposure:Control | Sex:F |
| SA096336 | C5 | No Exposure | Days Post Exposure:Control | Sex:F |
| SA096337 | C1 | No Exposure | Days Post Exposure:Control | Sex:M |
| SA096338 | C2 | No Exposure | Days Post Exposure:Control | Sex:M |
| SA096339 | C3 | No Exposure | Days Post Exposure:Control | Sex:M |
| SA096340 | C4 | No Exposure | Days Post Exposure:Control | Sex:M |
| SA096341 | D14_2 | Sonication | Days Post Exposure:Day Fourteen | Sex:F |
| SA096342 | D14_1 | Sonication | Days Post Exposure:Day Fourteen | Sex:F |
| SA096343 | D14_3 | Sonication | Days Post Exposure:Day Fourteen | Sex:F |
| SA096344 | D14_4 | Sonication | Days Post Exposure:Day Fourteen | Sex:F |
| SA096345 | D14_8 | Sonication | Days Post Exposure:Day Fourteen | Sex:M |
| SA096346 | D14_6 | Sonication | Days Post Exposure:Day Fourteen | Sex:M |
| SA096347 | D14_5 | Sonication | Days Post Exposure:Day Fourteen | Sex:M |
| SA096348 | D14_7 | Sonication | Days Post Exposure:Day Fourteen | Sex:M |
| SA096349 | D1_5 | Sonication | Days Post Exposure:Day One | Sex:F |
| SA096350 | D1_8 | Sonication | Days Post Exposure:Day One | Sex:F |
| SA096351 | D1_6 | Sonication | Days Post Exposure:Day One | Sex:F |
| SA096352 | D1_7 | Sonication | Days Post Exposure:Day One | Sex:F |
| SA096353 | D1_1 | Sonication | Days Post Exposure:Day One | Sex:M |
| SA096354 | D1_2 | Sonication | Days Post Exposure:Day One | Sex:M |
| SA096355 | D1_4 | Sonication | Days Post Exposure:Day One | Sex:M |
| SA096356 | D1_3 | Sonication | Days Post Exposure:Day One | Sex:M |
| SA096357 | D7_1 | Sonication | Days Post Exposure:Day Seven | Sex:F |
| SA096358 | D7_3 | Sonication | Days Post Exposure:Day Seven | Sex:F |
| SA096359 | D7_2 | Sonication | Days Post Exposure:Day Seven | Sex:F |
| SA096360 | D7_4 | Sonication | Days Post Exposure:Day Seven | Sex:F |
| SA096361 | D7_5 | Sonication | Days Post Exposure:Day Seven | Sex:M |
| SA096362 | D7_7 | Sonication | Days Post Exposure:Day Seven | Sex:M |
| SA096363 | D7_6 | Sonication | Days Post Exposure:Day Seven | Sex:M |
| SA096364 | D7_8 | Sonication | Days Post Exposure:Day Seven | Sex:M |
| Showing results 1 to 32 of 32 |
Collection:
| Collection ID: | CO001396 |
| Collection Summary: | 2 month old mice were exposed to sonication-induced trauma. Optic nerves were dissected from optic nerve head to optic chiasm. |
| Sample Type: | Neurons |
Treatment:
| Treatment ID: | TR001416 |
| Treatment Summary: | 4 groups of 8 mice, 4 males and 4 females per group. Control group was euthanized at 2 months. Other three groups were exposed to sonication to induce traumatic optic neuropathy. Then groups were euthanized at one day, seven days, and fourteen days, respectively. |
Sample Preparation:
| Sampleprep ID: | SP001409 |
| Sampleprep Summary: | Samples were homogenized and mitochondria were isolated. Lipids were extracted using butanol/methanol lipid extraction and run through mass spectrometer. |
Combined analysis:
| Analysis ID | AN002209 | AN002210 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Accela 600 | Thermo Accela 600 |
| Column | Thermo Acclaim C18 (150 x 2.1mm,3m) | Thermo Acclaim C18 (150 x 2.1mm,3m) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Main area | Main area |
Chromatography:
| Chromatography ID: | CH001620 |
| Instrument Name: | Thermo Accela 600 |
| Column Name: | Thermo Acclaim C18 (150 x 2.1mm,3m) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002055 |
| Analysis ID: | AN002209 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002056 |
| Analysis ID: | AN002210 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z. |
| Ion Mode: | POSITIVE |
