Summary of Study ST000464

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000356. The data can be accessed directly via it's Project DOI: 10.21228/M81K5Z This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000464
Study Title	Transpulmonary metabolomics in pulmonary arterial hypertension
Study Summary	We hypothesize that transpulmonary metabolomic profiling will demonstrate a PAH-specific metabolic signature. We will examine organ-specific metabolism by measuring blood flowing into (pulmonary artery) and out of (pulmonary artery wedge) the pulmonary circulation at the time of right heart catheterization (RHC). We will compare PAH to patients without PH and to a disease control cohort with PH due to left heart disease (pulmonary ventrical hypertension - PVH).
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-09-07
Raw Data Available	Yes
Raw Data File Type(s)	1r
Analysis Type Detail	NMR
Release Date	2018-10-10
Release Version	1

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Project:

Project ID:	PR000356
Project DOI:	doi: 10.21228/M81K5Z
Project Title:	Transpulmonary metabolomics in pulmonary arterial hypertension
Project Summary:	Pulmonary arterial hypertension (PAH) is a progressive and incurable disease characterized by obliteration of the pulmonary arterioles, elevated pulmonary vascular resistance (PVR), and eventual right heart failure and death. Current medical therapy for PAH is aimed at reducing PVR by targeting pathways involved in vasodilation. Pulmonary vasodilators improve functional capacity but do not target the underlying vascular obstruction. Despite multiple approved therapies, median survival after diagnosis is less than five years, indicating that additional therapeutic targets are needed.
Institute:	Vanderbilt University
Last Name:	Brittain
First Name:	Evan
Address:	2525 West End Ave, Suite 300, Nashville TN 37203
Email:	evan.brittain@Vanderbilt.Edu
Phone:	(615) 322-2318

Subject:

Subject ID:	SU000485
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Phenotype
SA023549	P_98	no PH \| Plasma_Type:PA
SA023550	P_124	no PH \| Plasma_Type:PA
SA023551	P_22	no PH \| Plasma_Type:PA
SA023552	P_10	no PH \| Plasma_Type:PA
SA023553	P_48	no PH \| Plasma_Type:PA
SA023554	P_30	no PH \| Plasma_Type:PA
SA023555	P_20	no PH \| Plasma_Type:PA
SA023556	P_18	no PH \| Plasma_Type:PA
SA023557	P_140	no PH \| Plasma_Type:PA
SA023558	P_68	no PH \| Plasma_Type:PA
SA023559	P_148	no PH \| Plasma_Type:PA
SA023560	P_8	no PH \| Plasma_Type:PA
SA023561	P_2	no PH \| Plasma_Type:PA
SA023562	P_60	no PH \| Plasma_Type:PA
SA023563	P_152	no PH \| Plasma_Type:PA
SA023564	P_132	no PH \| Plasma_Type:PA
SA023565	P_6	no PH \| Plasma_Type:PA
SA023566	P_97	no PH \| Plasma_Type:PCW
SA023567	P_139	no PH \| Plasma_Type:PCW
SA023568	P_21	no PH \| Plasma_Type:PCW
SA023569	P_19	no PH \| Plasma_Type:PCW
SA023570	P_29	no PH \| Plasma_Type:PCW
SA023571	P_1	no PH \| Plasma_Type:PCW
SA023572	P_47	no PH \| Plasma_Type:PCW
SA023573	P_5	no PH \| Plasma_Type:PCW
SA023574	P_17	no PH \| Plasma_Type:PCW
SA023575	P_67	no PH \| Plasma_Type:PCW
SA023576	P_131	no PH \| Plasma_Type:PCW
SA023577	P_123	no PH \| Plasma_Type:PCW
SA023578	P_147	no PH \| Plasma_Type:PCW
SA023579	P_151	no PH \| Plasma_Type:PCW
SA023580	P_7	no PH \| Plasma_Type:PCW
SA023581	P_9	no PH \| Plasma_Type:PCW
SA023423	P_76	PAH \| Plasma_Type:PA
SA023424	P_142	PAH \| Plasma_Type:PA
SA023425	P_12	PAH \| Plasma_Type:PA
SA023426	P_154	PAH \| Plasma_Type:PA
SA023427	P_116	PAH \| Plasma_Type:PA
SA023428	P_44	PAH \| Plasma_Type:PA
SA023429	P_86	PAH \| Plasma_Type:PA
SA023430	P_84	PAH \| Plasma_Type:PA
SA023431	P_80	PAH \| Plasma_Type:PA
SA023432	P_78	PAH \| Plasma_Type:PA
SA023433	P_14	PAH \| Plasma_Type:PA
SA023434	P_26	PAH \| Plasma_Type:PA
SA023435	P_88	PAH \| Plasma_Type:PA
SA023436	P_24	PAH \| Plasma_Type:PA
SA023437	P_52	PAH \| Plasma_Type:PA
SA023438	P_46	PAH \| Plasma_Type:PA
SA023439	P_96	PAH \| Plasma_Type:PA
SA023440	P_42	PAH \| Plasma_Type:PA
SA023441	P_54	PAH \| Plasma_Type:PA
SA023442	P_94	PAH \| Plasma_Type:PA
SA023443	P_56	PAH \| Plasma_Type:PA
SA023444	P_41	PAH \| Plasma_Type:PCW
SA023445	P_79	PAH \| Plasma_Type:PCW
SA023446	P_83	PAH \| Plasma_Type:PCW
SA023447	P_43	PAH \| Plasma_Type:PCW
SA023448	P_45	PAH \| Plasma_Type:PCW
SA023449	P_77	PAH \| Plasma_Type:PCW
SA023450	P_55	PAH \| Plasma_Type:PCW
SA023451	P_25	PAH \| Plasma_Type:PCW
SA023452	P_51	PAH \| Plasma_Type:PCW
SA023453	P_23	PAH \| Plasma_Type:PCW
SA023454	P_75	PAH \| Plasma_Type:PCW
SA023455	P_141	PAH \| Plasma_Type:PCW
SA023456	P_115	PAH \| Plasma_Type:PCW
SA023457	P_85	PAH \| Plasma_Type:PCW
SA023458	P_93	PAH \| Plasma_Type:PCW
SA023459	P_11	PAH \| Plasma_Type:PCW
SA023460	P_13	PAH \| Plasma_Type:PCW
SA023461	P_95	PAH \| Plasma_Type:PCW
SA023462	P_87	PAH \| Plasma_Type:PCW
SA023463	P_36	PVH \| Plasma_Type:PA
SA023464	P_58	PVH \| Plasma_Type:PA
SA023465	P_38	PVH \| Plasma_Type:PA
SA023466	P_62	PVH \| Plasma_Type:PA
SA023467	P_16	PVH \| Plasma_Type:PA
SA023468	P_66	PVH \| Plasma_Type:PA
SA023469	P_130	PVH \| Plasma_Type:PA
SA023470	P_104	PVH \| Plasma_Type:PA
SA023471	P_112	PVH \| Plasma_Type:PA
SA023472	P_32	PVH \| Plasma_Type:PA
SA023473	P_50	PVH \| Plasma_Type:PA
SA023474	P_34	PVH \| Plasma_Type:PA
SA023475	P_108	PVH \| Plasma_Type:PA
SA023476	P_110	PVH \| Plasma_Type:PA
SA023477	P_92	PVH \| Plasma_Type:PA
SA023478	P_156	PVH \| Plasma_Type:PA
SA023479	P_28	PVH \| Plasma_Type:PA
SA023480	P_114	PVH \| Plasma_Type:PA
SA023481	P_118	PVH \| Plasma_Type:PA
SA023482	P_138	PVH \| Plasma_Type:PA
SA023483	P_126	PVH \| Plasma_Type:PA
SA023484	P_122	PVH \| Plasma_Type:PA
SA023485	P_120	PVH \| Plasma_Type:PA
SA023486	P_128	PVH \| Plasma_Type:PA
SA023487	P_136	PVH \| Plasma_Type:PA
SA023488	P_82	PVH \| Plasma_Type:PA
SA023489	P_100	PVH \| Plasma_Type:PA

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Collection:

Collection ID:	CO000479
Collection Summary:	Plasma was collected from patients during right heart catheterization from the pulmonary artery (PA) and the pulmonary capillary wedge (PCW).
Sample Type:	Blood

Treatment:

Treatment ID:	TR000499
Treatment Summary:	None

Sample Preparation:

Sampleprep ID:	SP000492
Sampleprep Summary:	De-identified samples were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. Study samples were thawed on ice for sample preparation. A 150 µL aliquot of plasma was transferred to new labeled tubes for each study sample. A total study pool was generated by transferring 15 µL of plasma from each sample into a new 10 mL Falcon tube. The total pool sample was vortexed and 150 µL aliquots were transferred into 10 total pool-labeled tubes. Plasma and pooled samples were extracted with 450 µL methanol, vortexed for 2 min on a multi-tube vortexer, and centrifuged at 16,000 rcf for 5 min. A 400 µl aliquot of the supernatant was transferred into pre-labeled 2.0mL LoBind Eppendorf tubes, and the supernatant was lyophilized to complete dryness overnight. Samples were reconstituted with 250 µL of NMR master mix solution containing Chenomx ISTD: DSS-d6 and 0.20 M phosphate buffer at 7.4 pH. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 200uL aliquot of supernatants were transferred into a pre-labeled 3mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000492

Sampleprep Summary:

De-identified samples were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. Study samples were thawed on ice for sample preparation. A 150 µL aliquot of plasma was transferred to new labeled tubes for each study sample. A total study pool was generated by transferring 15 µL of plasma from each sample into a new 10 mL Falcon tube. The total pool sample was vortexed and 150 µL aliquots were transferred into 10 total pool-labeled tubes. Plasma and pooled samples were extracted with 450 µL methanol, vortexed for 2 min on a multi-tube vortexer, and centrifuged at 16,000 rcf for 5 min. A 400 µl aliquot of the supernatant was transferred into pre-labeled 2.0mL LoBind Eppendorf tubes, and the supernatant was lyophilized to complete dryness overnight. Samples were reconstituted with 250 µL of NMR master mix solution containing Chenomx ISTD: DSS-d6 and 0.20 M phosphate buffer at 7.4 pH. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 200uL aliquot of supernatants were transferred into a pre-labeled 3mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

Analysis ID:	AN000725
Analysis Type:	NMR
Num Factors:	7

NMR:

NMR ID:	NM000082
Analysis ID:	AN000725
Instrument Name:	Bruker Avance III
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	700 MHz