Summary of Study ST000416

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000325. The data can be accessed directly via it's Project DOI: 10.21228/M8788V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000416
Study TitleArmpit Biodiversity Metabolites and Odors
Study TypeTimecourse - 1 Day, 4 Day - No Deodorant/Antiperspirant, Deodorant, Antiperspirant
Study SummaryThe skin microbiota aid in the skin’s barrier function and help mature our immune system as well as produce odor by metabolizing sweat and other secretions produced by our bodies. Only now with the advancement of high throughput sequencing technologies has it become possible to sample the diversity of the microbiota that inhabits the outer surface of the body. This project will sample DNA from microbes (single celled organisms) that live on the human skin, investigate microbial gene regulation, skin surface metabolites, microbial odor production, and begin to assess the human perception of pleasantness of these microbial associated odors.
Institute
University of North Carolina
DepartmentRCMRC
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-16
Num Groups3
Total Subjects23
Num Males32
Num Females60
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2025-06-20
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8788V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000325
Project DOI:doi: 10.21228/M8788V
Project Title:Armpit Biodiversity Metabolites and Odors
Project Type:Metabolome Evaluation
Project Summary:Diverse communities of microscopic organisms (microbiota) live on the skin, and their collective genomes are referred to as the skin microbiome. The skin microbiota aid in the skin’s barrier function and help mature our immune system as well as produce odor by metabolizing sweat and other secretions produced by our bodies. Only now with the advancement of high throughput sequencing technologies has it become possible to sample the diversity of the microbiota that inhabits the outer surface of the body. This project will sample DNA from microbes (single celled organisms) that live on the human skin, investigate microbial gene regulation, skin surface metabolites, microbial odor production, and begin to assess the human perception of pleasantness of these microbial associated odors.
Institute:North Carolina Museum of Natural Sciences
Department:Natural Research Center
Laboratory:Genomics & Microbiology Research Laboratory
Last Name:Horvath
First Name:Julie
Address:121 W Jones Street, Raleigh, NC
Email:julie.horvath@naturalsciences.org
Phone:919-707-8242

Subject:

Subject ID:SU000437
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Age Range Gender
SA020840S_76Day1_antiperspirant 30-50 Female
SA020841S_30Day1_antiperspirant 30-50 Female
SA020842S_34Day1_antiperspirant 30-50 Female
SA020843S_29Day1_antiperspirant 30-50 Female
SA020844S_75Day1_antiperspirant 30-50 Female
SA020845S_45Day1_antiperspirant 30-50 Female
SA020846S_33Day1_antiperspirant 30-50 Female
SA020847S_46Day1_antiperspirant 30-50 Female
SA020848S_59Day1_antiperspirant 30-50 Male
SA020849S_63Day1_antiperspirant 30-50 Male
SA020850S_60Day1_antiperspirant 30-50 Male
SA020851S_64Day1_antiperspirant 30-50 Male
SA020852S_67Day1_antiperspirant <30 Female
SA020853S_72Day1_antiperspirant <30 Female
SA020854S_71Day1_antiperspirant <30 Female
SA020855S_21Day1_antiperspirant <30 Female
SA020856S_68Day1_antiperspirant <30 Female
SA020857S_9Day1_antiperspirant <30 Female
SA020858S_10Day1_antiperspirant <30 Female
SA020859S_22Day1_antiperspirant <30 Female
SA020860S_56Day1_antiperspirant <30 Male
SA020861S_55Day1_antiperspirant <30 Male
SA020862S_25Day1_antiperspirant >50 Female
SA020863S_26Day1_antiperspirant >50 Female
SA020864S_87Day1_deodorant 30-50 Male
SA020865S_88Day1_deodorant 30-50 Male
SA020866S_17Day1_deodorant <30 Female
SA020867S_18Day1_deodorant <30 Female
SA020868S_92Day1_deodorant <30 Male
SA020869S_91Day1_deodorant <30 Male
SA020870S_42Day1_deodorant >50 Male
SA020871S_41Day1_deodorant >50 Male
SA020872S_50Day1_noproduct 30-50 Female
SA020873S_38Day1_noproduct 30-50 Female
SA020874S_37Day1_noproduct 30-50 Female
SA020875S_83Day1_noproduct 30-50 Female
SA020876S_49Day1_noproduct 30-50 Female
SA020877S_84Day1_noproduct 30-50 Female
SA020878S_14Day1_noproduct <30 Female
SA020879S_13Day1_noproduct <30 Female
SA020880S_2Day1_noproduct >50 Female
SA020881S_1Day1_noproduct >50 Female
SA020882S_6Day1_noproduct >50 Male
SA020883S_80Day1_noproduct >50 Male
SA020884S_79Day1_noproduct >50 Male
SA020885S_5Day1_noproduct >50 Male
SA020886S_35Day4_antiperspirant 30-50 Female
SA020887S_48Day4_antiperspirant 30-50 Female
SA020888S_78Day4_antiperspirant 30-50 Female
SA020889S_36Day4_antiperspirant 30-50 Female
SA020890S_32Day4_antiperspirant 30-50 Female
SA020891S_47Day4_antiperspirant 30-50 Female
SA020892S_31Day4_antiperspirant 30-50 Female
SA020893S_77Day4_antiperspirant 30-50 Female
SA020894S_61Day4_antiperspirant 30-50 Male
SA020895S_62Day4_antiperspirant 30-50 Male
SA020896S_66Day4_antiperspirant 30-50 Male
SA020897S_65Day4_antiperspirant 30-50 Male
SA020898S_12Day4_antiperspirant <30 Female
SA020899S_11Day4_antiperspirant <30 Female
SA020900S_70Day4_antiperspirant <30 Female
SA020901S_74Day4_antiperspirant <30 Female
SA020902S_23Day4_antiperspirant <30 Female
SA020903S_24Day4_antiperspirant <30 Female
SA020904S_73Day4_antiperspirant <30 Female
SA020905S_69Day4_antiperspirant <30 Female
SA020906S_57Day4_antiperspirant <30 Male
SA020907S_58Day4_antiperspirant <30 Male
SA020908S_27Day4_antiperspirant >50 Female
SA020909S_28Day4_antiperspirant >50 Female
SA020910S_89Day4_deodorant 30-50 Male
SA020911S_90Day4_deodorant 30-50 Male
SA020912S_19Day4_deodorant <30 Female
SA020913S_20Day4_deodorant <30 Female
SA020914S_94Day4_deodorant <30 Male
SA020915S_93Day4_deodorant <30 Male
SA020916S_44Day4_deodorant >50 Male
SA020917S_43Day4_deodorant >50 Male
SA020918S_39Day4_noproduct 30-50 Female
SA020919S_40Day4_noproduct 30-50 Female
SA020920S_51Day4_noproduct 30-50 Female
SA020921S_52Day4_noproduct 30-50 Female
SA020922S_85Day4_noproduct 30-50 Female
SA020923S_86Day4_noproduct 30-50 Female
SA020924S_16Day4_noproduct <30 Female
SA020925S_15Day4_noproduct <30 Female
SA020926S_3Day4_noproduct >50 Female
SA020927S_4Day4_noproduct >50 Female
SA020928S_8Day4_noproduct >50 Male
SA020929S_81Day4_noproduct >50 Male
SA020930S_82Day4_noproduct >50 Male
SA020931S_7Day4_noproduct >50 Male
Showing results 1 to 92 of 92

Collection:

Collection ID:CO000431
Collection Summary:Cotton ball swiped each armpit for ~ 30 seconds
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR000451
Treatment Summary:No Deodorant/Antiperspirant wearers, Deodorant wearers, Antiperspirant wearers

Sample Preparation:

Sampleprep ID:SP000444
Sampleprep Summary:Extracted sweat from cotton balls were mixed with 2mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Combined analysis:

Analysis ID AN000658
Chromatography ID CH000475
MS ID MS000584
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus 4D GC
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH000475
Instrument Name:Leco Pegasus 4D GC
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Pressure:12.8psi
Flow Gradient:constant flow
Flow Rate:1ml/min
Injection Temperature:250C
Retention Index:FAME markers C8-C30
Chromatography Type:GC

MS:

MS ID:MS000584
Analysis ID:AN000658
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
Ion Mode:POSITIVE
  logo