Summary of Study ST000824
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000586. The data can be accessed directly via it's Project DOI: 10.21228/M8XD4X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000824 |
Study Title | 2HG concentration in human IDH1m cells |
Study Type | MS analysis |
Study Summary | SJGBM2 and MGG8 glioma cells were stable transfected with IDH1-R132H mutated. The expression of IDH1-R132H was confirmed by Western Blot assay. The aim of this project is analyze the production of 2HG in the stable transfected cells (SJGBM-IDH1m and MGG8-IDH1m) compared with the control group WT. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2017-07-14 |
Num Groups | 4 |
Total Subjects | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2019-01-22 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN001310 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent |
Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490A QQQ |
Ion Mode | NEGATIVE |
Units | uM/ng protein |