Summary of Study ST002749
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001712. The data can be accessed directly via it's Project DOI: 10.21228/M8DM8V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002749 |
| Study Title | Bromeliad lipidomics for adaptation to elevation |
| Study Summary | Untargeted analysis on Bromeliades leaf samples. The aim of this study was to evaluate the lipid profile alterations on Pitcairnia flammea leaves based on the different altitudes where they were collected. A lipidomic approach was applied to the samples. Ultra-high performance liquid chromatography coupled to electrospray ionization mass spectrometry (UHPLC-ESI-MS) was used to acquire raw data and MS-DIAL was used to perform data preprocessing. The statistical analysis of UHPLC-ESI-MS data in both ionization modes enabled the visualization of a trend distribution based on the altitude. Our study grouped the individuals into three major groups: one with individuals from the lower elevations (UBA, RAN, and COR), another with individuals from the highest elevations (PAP and MAR), and another with individuals from mixed localities from intermediate elevations. Higher elevation population showed an increase in very long chain fatty acids and monounsaturated lipids compared to lower elevation as a response to cold higher elevations affecting the leaves' membrane fluidity. In addition, the higher elevation population showed a higher abundance of hexosylceramides and phosphatidylglycerol compared to lower ones. |
| Institute | University of Campinas |
| Department | Chemistry's Institute |
| Laboratory | Laboratory of Bioanalytics and Integrated Omics |
| Last Name | Matos |
| First Name | Taynara |
| Address | Rua Josué de Castro, s/n – Cidade Universitária, 13083-970, Campinas – SP, Brazil |
| t262827@dac.unicamp.br | |
| Phone | (85)996154192 |
| Submit Date | 2023-06-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-08-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004459 | AN004460 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system | Thermo Scientific UltiMate™ 3000 UHPLC RSLCnano system |
| Column | Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um) | Supelco Sigma-Aldrich Titan C18 (100 x 2.1mm, 1.9um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Peak area | Peak area |
