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MB Sample ID: SA005432
Local Sample ID: | S17C |
Subject ID: | SU000115 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | SKOV3ip1 |
Subject Comments: | p80, p85, p86, p89 |
Cell Passage Number: | p80, p85, p86, p89 |
Cell Counts: | p80, p85, p86, p89 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000152 | AN000153 | AN000154 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | |||
Chromatography system | |||
Column | |||
MS Type | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap |
Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
Units | Area % | nM | nM |
Chromatography:
Chromatography ID: | CH000108 |
Chromatography Summary: | Oxylipin analysis |
Methods Filename: | Newman_Lab_Oxylipin_Extraction_&_Analysis_Protocol_Lengyel_WCMC_P&F_18hr.pdf Newman_Lab_Oxylipin_Extraction_&_Analysis_Protocol_Lengyel_WCMC_P&F_4hr.pdf |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 16 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |