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MB Sample ID: SA006126
Local Sample ID: | D-36 |
Subject ID: | SU000134 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 13-wk-old |
Gender: | Male |
Species Group: | Mammal |
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Combined analysis:
Analysis ID | AN000195 | AN000196 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | ||
Chromatography system | ||
Column | ||
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex API 5000 QQQ | Thermo TSQ Quantum Ultra |
Ion Mode | NEGATIVE | POSITIVE |
Units | uM | uM |
Chromatography:
Chromatography ID: | CH000129 |
Chromatography Summary: | Plasma free fatty acid concentrations were measured by liquid chromatography/mass spectrometry (LC/MS), as described previously (51). Briefly, 50 ?l of plasma was spiked with heptadecanoate internal standard (ISTD) and analyzed with Applied Biosystems (Foster City, CA) API5000 mass spectrometer coupled with a Cohesive (Franklin, MA) TX2 liquid chromatography system. Concentration of individual FFA was measured against a six-point standard curve prepared for each analyte. Both the ISTD and individual FFA standard curves were prepared in 2% fatty acid-free human albumin solution. All analytes were monitored as their [M ? H]? ions. LCFa-CoA esters were estimated using the LC-MS/MS method (9). After extraction in the presence of internal standard (20 ng of heptadecanoyl-CoA), samples were analyzed by UHPLC-ESI-MS/MS operating in multiple reaction monitoring mode [Waters Acquity UHPLC, C8 UPLC BEH column 2.1 150 mm, 1.7 ?m (Waters, Milford, MA) and TSQ Quantum Ultra triple-quad mass spectrometer (Thermo Fisher Scientific, Waltham, MA)]. All standard curves were prepared using chemicals from Avanti Polar Lipids. |
Methods Filename: | PMID-24368672-Zabielski-Nair-AJPEM-2014.pdf |
Chromatography Comments: | Pubmed ID: 24368672 |