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MB Sample ID: SA012392
Local Sample ID: | 455 |
Subject ID: | SU000304 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 18-88 |
Gender: | Femal(116), Male(118) |
Human Smoking Status: | 67 nonsmokers and 167 smokers |
Human Alcohol Drug Use: | 14 no alcohol and 220 alcohol |
Species Group: | Human |
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Combined analysis:
Analysis ID | AN000452 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1260 |
Column | SeQuant ZIC-cHILIC |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | UNSPECIFIED |
Units | cps |
Chromatography:
Chromatography ID: | CH000323 |
Chromatography Summary: | The LC system was composed of two Agilent 1260 binary autosampler, and Agilent 1290 column valve (Agilent 10 ?L for analysis using negative ionization mode and 2 positive ionization mode. Both chromatographic hydrophilic interaction ZIC-cHILIC KGaA, to perform the separation, while the other column for the next injection. The flow temperature was kept the phase was composed of Solvents A (5 mM acetonitrile +0.2% acetic acetonitrile/ confirmed by spiking the with metabolites). However, some metabolites that could had similar m/z values (<1 Da) were acid and 3- samples not undergo any significant shift (each peak was of analysis), which proved the |
Instrument Name: | Agilent 1260 |
Column Name: | SeQuant ZIC-cHILIC |
Column Temperature: | 40oC |
Flow Gradient: | 0-2 min: 90%B; 2-5 min: to 50%B; 5-9 min: 50%B |
Flow Rate: | 0.3 mL/min |
Injection Temperature: | 4oC |
Sample Injection: | 2 uL for positive and 10 uL for negative |
Solvent A: | 90% water/10% acetonitrile; 0.2% acetic acid; 5 mM ammonium acetate |
Solvent B: | 90% acetonitrile/ 10% water; 0.2% acetic acid; 5 mM ammonium acetate |
Analytical Time: | 18 min |
Capillary Voltage: | 3.8kV |
Chromatography Type: | HILIC |