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MB Sample ID: SA022651
Local Sample ID: | UNIFESP_lip_LF22 |
Subject ID: | SU000466 |
Subject Type: | Human patients (Women) |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | <= 37 years |
Gender: | Female |
Species Group: | Human |
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Combined analysis:
Analysis ID | AN000696 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity I-Class |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Waters Synapt G2 S QTOF |
Ion Mode | POSITIVE |
Units | m/z intensity |
Chromatography:
Chromatography ID: | CH000504 |
Chromatography Summary: | Reversed-phase analysis was performed on a Waters Ultra Performance liquid chromatography Acquity I-Class system using an Acquity HSS T3 2.10 x 100 mm column (Waters, Milford, USA). The column was maintained at 55 °C. The lipid extracts were analyzed by UPLC-MSE by injecting 10 μl of each sample dissolved in 1 mL of water:acetonitrile:2-propanol (H2O:ACN:2-propanol; 1:1:2, v:v:v) (Merck, Darmstadt, Germany). The chromatographic run was carried out in gradient mode with a flow rate of 0.5 mL min-1 with initial composition of 50% solvent B (ACN:2-propanol; 10:90) in solvent A (H2O:ACN; 40:60, v:v + 10 mM ammonium acetate (NH4Ac) (Sigma-Aldrich, Saint Louis, USA), maintained for 0 to 0.5 min. The gradient composition was changed linearly from 50% to 100% solvent B between 0.5 to 4.5 minutes, returning to the initial composition of 50% B in 4.6 minutes, kept to 5.0 minutes. |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 55ºC |
Flow Rate: | 0.5 mL.min-1 |
Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/90% isopropanol |
Chromatography Type: | Reversed phase |