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MB Sample ID: SA027265
| Local Sample ID: | 20150724_YU_1_1_22 |
| Subject ID: | SU000544 |
| Subject Type: | Animal |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
| Species Group: | Fish |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000796 | AN000797 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters 2777C | Waters 2777C |
| Column | Waters Acquity BEH C18 (150 x 2mm,1.7um) | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 XS QTOF | Waters Synapt G2 XS QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak intensity | peak intensity |
Chromatography:
| Chromatography ID: | CH000572 |
| Chromatography Summary: | Chromatographic separation was performed on a ACQUITY UPLC BEH C18 analytical column (100×2.1 mm, 1.7 µm, Waters, Milford, USA) using a 2777C UPLC system (Waters). The flow rate was 0.40 mL/min and the mobile phase was composed of solvents A (95% H2O/5% acetonitrile + 0.1% formic acid) and B (95% acetonitrile/5% H2O＋0.1% formic acid).The gradient program was optimized as follows: 0–0.1 min, 100% A; 0.1–0.6 min, 100% A-50% A; 0.6–5 min, 50%–0% A; 5–8 min, 0% A; 8–10 min, 0% A-100% A. Injection volume was 10 µl. The eluent from the column was directly added in to the mass spectrometer without split. |
| Instrument Name: | Waters 2777C |
| Column Name: | Waters Acquity BEH C18 (150 x 2mm,1.7um) |
| Flow Gradient: | 100% acetonitrile |
| Flow Rate: | 0.40 mL/min |
| Solvent A: | 95% water/5% acetonitrile; 0.1% formic acid |
| Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
