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MB Sample ID: SA028145
Local Sample ID: | R2_60exp3 |
Subject ID: | SU000568 |
Subject Type: | Cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Genotype Strain: | Cam3.IIR539T (Cambodian isolate, artemisinin resistant) R1 | Cam3.IIC580Y (Cambodian isolate, artemisinin resistant) R2 | Cam3.IIrev (Cambodian isolate, artemisinin sensitive) S | uninfected red blood cells |
Cell Strain Details: | Artemisinin resistant strain (R1) | Artemisinin resistant strain (R2) | Artemisinin sensitive strain (S) | uninfected red blood cells |
Cell Primary Immortalized: | Primary | Primary | Primary | Primary |
Cell Counts: | 5 x 10e7 per sample (purified infected cells) | 5 x 10e7 per sample (purified infected cells) | 5 x 10e7 per sample (purified infected cells) | 5 x 10e7 per sample |
Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000832 | AN000833 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | ZIC-pHILIC (Merck Sequant) | ZIC-pHILIC (Merck Sequant) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | Peak height |
Chromatography:
Chromatography ID: | CH000595 |
Chromatography Summary: | Untargeted HILIC method |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | ZIC-pHILIC (Merck Sequant) |
Column Temperature: | 25°C |
Flow Gradient: | linear gradient -time, %B as follows: 0min- 80%, 15min- 50%, 18min- 5%, 21min- 5%, 24min- 80%, 32min- 80%. |
Flow Rate: | 300 μL/min |
Injection Temperature: | 4 °C |
Internal Standard: | internal standards (CHAPS, CAPS, PIPES and TRIS; all at 1 µM) |
Sample Injection: | 10 μL |
Solvent A: | 100% water; 20 mM ammonium carbonate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |