Return to study ST000882 main page
MB Sample ID: SA051167
| Local Sample ID: | S8 |
| Subject ID: | SU000916 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 13 - 66 |
| Gender: | Female and Male |
| Human Inclusion Criteria: | Retrospective serum samples from leprosy patients diagnosed or not with type 1 reaction that were not receiving multidrug therapy or corticosteroid therapy at the time of blood collection. |
| Human Exclusion Criteria: | Retrospective serum samples from leprosy patients that were under multidrug therapy and/or corticosteroid therapy at the time of blood collection were excluded from the study. |
| Subject Comments: | The patients exhibited borderline tuberculoid, borderline borderline and borderline lepromatous forms of leprosy and was diagnosed or not with type 1 reaction. |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001438 | AN001439 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Waters Xbridge BEH C18 XP (100 x 2.1mm,2.5um) | Waters Xbridge BEH C18 XP (100 x 2.1mm,2.5um) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | TOF |
| MS instrument name | Agilent 6224 TOF | Agilent 6224 TOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH001009 |
| Chromatography Summary: | This was used for negative mode. The solvent gradient used for all chromatography was 2% solvent B for 3 min followed by a 15 min linear gradient of 2% to 98% solvent B, which was held for 0.3 min. Then returned to starting conditions over 1.2 min. Triplicate LC-MS injections were made of each sample and the order of sample injection (including triplicates) was randomized. |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters Xbridge BEH C18 XP (100 x 2.1mm,2.5um) |
| Column Temperature: | 50C |
| Flow Gradient: | 0.25 mL/min |
| Flow Rate: | 0.25 mL/min |
| Injection Temperature: | 4C |
| Sample Injection: | 20uL |
| Solvent A: | 89% water/5% acetonitrile/5% isopropanol; 5 mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 5 mM ammonium acetate |
| Capillary Voltage: | 2.8 kV |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001010 |
| Chromatography Summary: | This was used for positive mode. The solvent gradient used for all chromatography was 2% solvent B for 3 min followed by a 15 min linear gradient of 2% to 98% solvent B, which was held for 0.3 min. Then returned to starting conditions over 1.2 min. Triplicate LC-MS injections were made of each sample and the order of sample injection (including triplicates) was randomized. |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters Xbridge BEH C18 XP (100 x 2.1mm,2.5um) |
| Column Temperature: | 50C |
| Flow Gradient: | 0.25 mL/min |
| Flow Rate: | 0.25 mL/min |
| Injection Temperature: | 4C |
| Sample Injection: | 20uL |
| Solvent A: | 95% water/5% acetonitrile; 0.1% formic acid |
| Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid |
| Capillary Voltage: | 4.0kV |
| Chromatography Type: | Reversed phase |
