Return to study ST000924 main page
MB Sample ID: SA055254
Local Sample ID: | 31 |
Subject ID: | SU000962 |
Subject Type: | Cell culture |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Cell Strain Details: | C57BL/6 |
Cell Primary Immortalized: | AT1 |
Cell Counts: | ~60% confluency 6 well plate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001517 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | GCs connected in series are both from J&W/Agilent (part 122-5512) |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975 |
Ion Mode | POSITIVE |
Units | Peak values (Log transformed) |
Chromatography:
Chromatography ID: | CH001070 |
Chromatography Summary: | Metabolites were made volatile with trimethylsilyl (TMS) groups using N-methyl- N-(trimethylsilyl) trifluoroacetamide or MSTFA with catalytic trimethylchlorosilane at 50 C. GC/MS methods generally follow those of Roessner et al. (Roessner et al. 2000), Fiehn et al. (2008), and Kind et al. (2009), and used a 6890N GC connected to a 5,975 inert single-quadrupole MS (Agilent Technologies, Santa Clara, CA). The two wall-coated, open-tubular (WCOT) GC columns connected in series are both from J&W/Agilent (part 122-5512), DB5- MS, 15 m in length, 0.25 mm in diameter, with an 0.25-lm luminal film. Positive ions generated with conventional electron-ionization (EI) at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time. |
Instrument Name: | Agilent 6890N |
Column Name: | GCs connected in series are both from J&W/Agilent (part 122-5512) |
Chromatography Type: | GC |