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MB Sample ID: SA072425
Local Sample ID: | HA2_16 |
Subject ID: | SU001117 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Tcf/LacZ mice |
Age Or Age Range: | 6-8 weeks |
Gender: | Male and female |
Animal Inclusion Criteria: | The Tcf/LacZ strain was previously backcrossed onto the C3H background C3H background, and confirmed by PCR to lack the rd1 mutation |
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Combined analysis:
Analysis ID | AN001755 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Accela 600 |
Column | Thermo Acclaim C30 (150 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | relative abundance (main area) |
Chromatography:
Chromatography ID: | CH001239 |
Chromatography Summary: | Reversed phase chromatographic separation utilized Accela Autosampler (Thermo), Accela 600 pump (Thermo) and Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo). The column temperature was maintained at 30˚C and tray temperature at 20˚C. Solvent A was composed of 10 mM ammonium acetate (LC-MS grade) in 60:40 methanol:water (LC-MS grade) with 0.2% formic acid (FA; LC-MS grade). Solvent B was composed of 10 mM ammonium acetate with 60:40 methanol:chloroform with 0.2% FA. The flow rate was 260 µL/min and injection volume was 10 µL. The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min. |
Instrument Name: | Thermo Accela 600 |
Column Name: | Thermo Acclaim C30 (150 x 2.1mm,3um) |
Column Temperature: | 30˚C |
Flow Gradient: | The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min. |
Flow Rate: | 260 µL/min |
Sample Injection: | 10 µL |
Solvent A: | 60% methanol/40% water; 0.2% formic acid; 10 mM ammonium acetate |
Solvent B: | 60% methanol/40% chloroform; 0.2% formic acid; 10 mM ammonium acetate |
Analytical Time: | 20 min |
Washing Buffer: | methanol |
Chromatography Type: | Reversed phase |