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MB Sample ID: SA074527
| Local Sample ID: | X01222018_Colo320_20V_Lipid_POS_14 |
| Subject ID: | SU001137 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | ALDH1A1 |
| Cell Passage Number: | 5 |
| Cell Counts: | 4 million |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001779 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters |
| Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 XS QTOF |
| Ion Mode | POSITIVE |
| Units | abundance corresponding to m/z |
Chromatography:
| Chromatography ID: | CH001257 |
| Chromatography Summary: | The mobile phase involved a gradient comprising varying amounts of solutions A (10 mM ammonium formate in 60% v/v acetonitrile) and B (10 mM ammonium formate in 95% isopropanol and 5% acetonitrile). The linear gradient elution was: 40–43% B (0–2 min), 43–50% B (over 0.1 min), 50–54% B (over 10 min), 54–70% B (over 0.1 min), 70–99% B (over 5.9 min), 99-40% B (over 0.1 min), and 40% B (for 2 min), run at 300 μl/min in positive Electrospray Ionization mode and the column temperature was maintained at 55 °C |
| Instrument Name: | Waters |
| Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| Flow Rate: | 300 μl/min |
| Injection Temperature: | 4 |
| Internal Standard: | SPLASH™ Lipidomix® Mass Spec Standard (Avanti Polar Lipids, Inc.) |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 95% isopropanol/5% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
