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MB Sample ID: SA083278

Local Sample ID:17-Synechocystis_6803-cell-8a-2
Subject ID:SU001264
Subject Type:Bacteria
Subject Species:Synechocystis sp. PCC 6803
Taxonomy ID:1148
Genotype Strain:NCBI:txid1148
Cell Biosource Or Supplier:ATCC

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Combined analysis:

Analysis ID AN001993
Analysis type MS
Chromatography type GC
Chromatography system Thermo ISQ
Column Trace 1310 GC
MS Type EI
MS instrument type GC-TOF
MS instrument name Thermo ISQ
Ion Mode POSITIVE
Units spectral abundance per cell

Chromatography:

Chromatography ID:CH001441
Chromatography Summary:For non-targeted GC-MS experiments, metabolites were detected using a Trace 1310 GC coupled to a Thermo ISQ mass spectrometer. Samples (1 µL) were injected at a 10:1 split ratio to a 30 m TG-5MS column (Thermo Scientific, 0.25 mm i.d., 0.25 μm film thickness) with a 1.2 mL/min helium gas flow rate. GC inlet was held at 285°C. The oven program started at 140°C for 1 min, followed by a ramp of 15°C/min to 330°C, and 5 min hold. Masses between 50-650 m/z were scanned at 5 scans/sec under electron impact ionization. Transfer line and ion source were held at 300 and 260°C, respectively. Pooled QC samples were injected after every 6 actual samples.
Instrument Name:Thermo ISQ
Column Name:Trace 1310 GC
Chromatography Type:GC
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