Return to study ST001199 main page
MB Sample ID: SA083505
| Local Sample ID: | QC8 |
| Subject ID: | SU001266 |
| Subject Type: | Bacteria |
| Subject Species: | Synechocystis sp. PCC 6803 |
| Taxonomy ID: | 1148 |
| Genotype Strain: | NCBI:txid1148 |
| Cell Biosource Or Supplier: | ATCC |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001995 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Xevo G2 |
| Column | Waters Acquity UPLC CSH Phenyl Hexyl ( 100 x 1.0mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Xevo QS |
| Ion Mode | POSITIVE |
| Units | spectral abundance per cell |
Chromatography:
| Chromatography ID: | CH001443 |
| Chromatography Summary: | For non-targeted LC-MS experiments, two microliters of extract were injected onto a Waters Acquity UPLC system in discrete, randomized blocks with a pooled QC injection after every 6 sample injections and separated using a Waters Acquity UPLC CSH Phenyl Hexyl column (1.7 µM, 1.0 x 100 mm), using a gradient from solvent A (2mM ammonium hydroxide, 0.1% formic acid) to solvent B (Acetonitrile, 0.1% formic acid). Injections were made in 100% A, held at 100% A for 1 min, ramped to 98% B over 12 minutes, held at 98% B for 3 minutes, and then returned to starting conditions over 0.05 minutes and allowed to re-equilibrate for 3.95 minutes, with a 200 µL/min constant flow rate. The column and samples were held at 65 °C and 6 °C, respectively. The column eluent was infused into a Waters Xevo G2 Q-TOF-MS with an electrospray source in positive mode, scanning 50-2000 m/z at 0.2 seconds per scan, alternating between MS (6 V collision energy) and MSE mode (15-30 V ramp). Calibration was performed using sodium iodide with 1 ppm mass accuracy. The capillary voltage was held at 2200 V, source temp at 150 °C, and nitrogen desolvation temp at 350 °C with a flow rate of 800 L/hr. |
| Instrument Name: | Waters Xevo G2 |
| Column Name: | Waters Acquity UPLC CSH Phenyl Hexyl ( 100 x 1.0mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | Injections were made in 100% A, held at 100% A for 1 min, ramped to 98% B over 12 minutes, held at 98% B for 3 minutes, and then returned to starting conditions over 0.05 minutes and allowed to re-equilibrate for 3.95 minutes |
| Flow Rate: | 200ul/min |
| Solvent A: | 100% water; 0.1% formic acid; 2 mM ammonium hydroxide |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
