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MB Sample ID: SA096541
Local Sample ID: | Nip-0h-1 |
Subject ID: | SU001405 |
Subject Type: | Plant |
Subject Species: | Oryza sativa Japonica Group |
Taxonomy ID: | 39947 |
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Combined analysis:
Analysis ID | AN002219 | AN002220 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Waters CORTECS C18 (150 x 2.1mm,2.7um) | Waters CORTECS C18 (150 x 2.1mm,2.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Relative content | Relative content |
Chromatography:
Chromatography ID: | CH001628 |
Chromatography Summary: | Five μL of each sample was eluted using a CORTECS® C18 column (150 mm × 2.1 mm, 2.7μm, Waters) with 0.4 mL/min flow rate. The mobile phase A was water: ACN (40: 60, v/v) with 0.1% formic acid and 10mM ammonium formate, and the mobile phase B was 2-propanol: ACN (90:10, v/v) with 0.1% formic acid and 10mM ammonium formate. The compounds were separated by a elution gradient: 20% B was firstly maintained for 0.2 min, then linearly decreased to 60% B from 0.2 to 2min, to 100% B from 2 to 9min, and maintained at 100% B from 9 to 10 min, then linearly increased to 20% B from 10 to 10.5 min followed by equilibration at 20% B for 3.5 min. |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters CORTECS C18 (150 x 2.1mm,2.7um) |
Flow Rate: | 0.4 mL/min |
Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Chromatography Type: | Reversed phase |