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MB Sample ID: SA098897
Local Sample ID: | VV_16_HEpG2_SDC1 |
Subject ID: | SU001433 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | HPACC |
Cell Passage Number: | 10-20 |
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Combined analysis:
Analysis ID | AN002263 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Ultimate 3000 |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Normalised peak area |
Chromatography:
Chromatography ID: | CH001662 |
Chromatography Summary: | The mobile phases consisted of 60:40 ACN: H2O with 10mM ammonium formate, 0.1% formic acid and 5µM of phosphoric acid (A) and 90:10 IPA:ACN with 10 mM ammonium formate, 0.1% formic acid and 5µM phosphoric acid (B). The gradient was as follows: 0-2 min 30% (B); 2-8 min 50% (B); 8-15 min 99% (B), 15-16 min 99% (B), 16-17 min 30% (B). Sample temperature was maintained at 6°C in the autosampler and 5 µL of sample were injected into the LC-MS instrument. |
Instrument Name: | Ultimate 3000 |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Flow Gradient: | 0-2 min 30% (B); 2-8 min 50% (B); 8-15 min 99% (B), 15-16 min 99% (B), 16-17 min 30% (B). |
Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10mM ammonium formate; 5µM phosphoric acid |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate;5µM phosphoric acid |
Chromatography Type: | Reversed phase |