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MB Sample ID: SA114366
| Local Sample ID: | PL25_Lipids_40970 |
| Subject ID: | SU001482 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002352 | AN002353 | AN002354 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity UPLC | Waters Acquity UPLC | Waters Acquity UPLC |
| Column | Waters Acquity UPLC BEH amide,(100 x 2.1mm,1.7um,100 Å) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um,100 Å) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um,100 Å) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF |
| MS instrument name | Waters Xevo G2-XS | Waters Xevo G2-XS | Waters Xevo G2-XS |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE |
| Units | normalized ion abundance | normalized ion abundance | normalized ion abundance |
Chromatography:
| Chromatography ID: | CH001724 |
| Chromatography Summary: | Chromatographic separation was performed using HILIC (Acquity™ UPLC BEH amide, 100 Å, 1.7 µm 2.1× 100mm, Waters Corporation, Milford, U.S.A). Quaternary solvent system mobile phases were (A) 0.1% formic acid in water, (B) 0.1% formic acid in acetonitrile and (D) 100mM ammonium formate, pH 3. For HILIC separation, a starting gradient of 95% B and 5% D was increase linearly to 70% A, 25% B and 5% D over a 5min period at 0.4mL/min flow rate, followed by 1 min isocratic gradient at 100 % A at 0.4mL/min flow rate.Waters Acquity™ UPLC system with 2D column regeneration configuration |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC BEH amide,(100 x 2.1mm,1.7um,100 Å) |
| Flow Gradient: | a starting gradient of 95% B and 5% D was increase linearly to 70% A, 25% B and 5% D over a 5min period followed by 1 min isocratic gradient at 100% A |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 100% water; 0.1% formic acid(A), 100% acetonitrile; 0.1% formic acid(B), 100% water; 100 mM ammonium formate, pH 3(D) |
| Solvent B: | 100% water; 0.1% formic acid(A), 100% acetonitrile; 0.1% formic acid(B), 100% water; 100 mM ammonium formate, pH 3(D) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001725 |
| Chromatography Summary: | Chromatographic separation was performed using C18 (Acquity™ UPLC HSS T3, 100 Å, 1.8 µm,, 2.1×100mm, Water Corporation, Milford, U.S.A) columns at 45°C. Quaternary solvent system mobile phases were (A) 0.1% formic acid in water, (B) 0.1% formic acid in acetonitrile and (D) 100mM ammonium formate, pH 3. For C18 separation, a chromatography gradient of was as follows: starting conditions, 100% A, with linear increase to final conditions of 5% A, 95% B followed by isocratic gradient at 95% B, 5% D for 1 min.Waters Acquity™ UPLC system with 2D column regeneration configuration |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um,100 Å) |
| Column Temperature: | 45 |
| Flow Gradient: | starting conditions, 100% A, with linear increase to final conditions of 5% A, 95% B followed by isocratic gradient at 95% B, 5% C for 1 min. |
| Solvent A: | 100% water; 0.1% formic acid(A); 100% acetonitrile; 0.1% formic acid(B); 100 mM ammonium formate, pH 3(C) |
| Solvent B: | 100% water; 0.1% formic acid(A); 100% acetonitrile; 0.1% formic acid(B); 100 mM ammonium formate, pH 3(C) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001726 |
| Chromatography Summary: | Chromatographic separation was performed using a C18 (Acquity™ UPLC HSS T3, 100 Å, 1.8 µm, 2.1×100mm, Water Corporation, Milford, U.S.A) column at 55°C. The mobile phases were (A) water, (B) Acetonitrile, (C) 2-propanol and (D) 500mM ammonium formate, pH 3. A starting elution gradient of 20% A, 30% B, 49% C and 1% D was increased linearly to 10% B, 89% C and 1 % D for 5.5 min, followed by isocratic elution at 10% B, 89%C and 1%D for 1.5 min and column equilibration with initial conditions for 1min. Waters Acquity™ UPLC system with 2D column regeneration configuration |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um,100 Å) |
| Column Temperature: | 55 |
| Flow Gradient: | A starting elution gradient of 20% A, 30% B, 49% C and 1% D was increased linearly to 10% B, 89% C and 1 % D for 5.5 min, followed by isocratic elution at 10% B, 89%C and 1%D for 1.5 min and column equilibration with initial conditions for 1min. |
| Solvent A: | 100% water(A), 100% acetonitrile(B), 100% isopropanol(C), 500 mM ammonium formate, pH 3(D) |
| Solvent B: | 100% water(A), 100% acetonitrile(B), 100% isopropanol(C), 500 mM ammonium formate, pH 3(D) |
| Chromatography Type: | Reversed phase |
