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MB Sample ID: SA120853
| Local Sample ID: | 514 |
| Subject ID: | SU001504 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002391 | AN002392 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001758 |
| Chromatography Summary: | Chromatographic conditions RPLC separation was performed using Zorbax SB columns (2.1 X 50mm, 1.8 Micron, 600 Bar; 827700-914) purchased from Agilent Technologies (Santa Clara, CA, USA). Mobile phases for RPLC consisted of 0.06% acetic acid in water (phase A) and 0.06% acetic acid in MeOH (phase B). Metabolites were eluted from the column at a flow rate of 0.6 mL/min, leading to a backpressure of 220– 280 bar at 99% phase A. A linear 1%–80% phase B gradient was applied over 9–10 min. The oven temperature was set to 60C, and the sample injection volume was 5 mL. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Chromatography Type: | Reversed phase |
