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MB Sample ID: SA121578
| Local Sample ID: | SCFA_S12 |
| Subject ID: | SU001505 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002393 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Ion trap |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | nM |
Chromatography:
| Chromatography ID: | CH001759 |
| Chromatography Summary: | Water and HPLC-grade isopropanol, both acidified with 0.1% formic acid were used as mobile phases A and B respectively. Chromatographic separation was achieved using an Acquity UPLC HSS T3 1.8 M, 2.1 mm x 100 mm column at a flow rate of 0.35 mL/min under the following condition: isocratic 15% B (0.00 − 2.00 min), linear gradient 15% to 33% B (2.01–6.00 min), linear gradient 33% to 34% B (6.01–7.50 min), linear gradient 34% to 36% B (7.51–12.00 min), isocratic 100% B (12.01–13.00 min), isocratic 15% B (13.01–15.00 min). Using an autosampler thermostatted at 4 ◦C, 1 L of each sample was injected onto the column maintained at 50 ◦C. The needle was flushed with methanol postinjection to minimize carry-over effect. |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Flow Gradient: | isocratic 15% B (0.00 - 2.00 min), linear gradient 15% to 33% B (2.01-6.00 min), linear gradient 33% to 34% B (6.01-7.50 min), linear gradient 34% to 36% B (7.51-12.00 min), isocratic 100% B (12.01-13.00 min), isocratic 15% B (13.01-15.00 min). |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% isopropanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
