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MB Sample ID: SA123901

Local Sample ID:166
Subject ID:SU001524
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN002421 AN002422 AN002423 AN002424
Analysis type MS MS MS MS
Chromatography type HILIC HILIC HILIC HILIC
Chromatography system Agilent 1260 Infinity Agilent 1260 Infinity Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6520 QTOF Agilent 6520 QTOF Agilent 6545 QTOF Agilent 6545 QTOF
Ion Mode POSITIVE POSITIVE POSITIVE POSITIVE
Units Peak area Peak area Peak area Peak area

Chromatography:

Chromatography ID:CH001778
Chromatography Summary:The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC.
Instrument Name:Agilent 1260 Infinity
Column Name:Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um)
Column Temperature:35
Flow Gradient:100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column.
Flow Rate:0.3 mL/min
Solvent A:95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Solvent B:5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Chromatography Type:HILIC
  
Chromatography ID:CH001779
Chromatography Summary:The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC.
Instrument Name:Agilent 1260 Infinity
Column Name:Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um)
Column Temperature:35
Flow Gradient:100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column.
Flow Rate:0.3 mL/min
Solvent A:95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Solvent B:5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Chromatography Type:HILIC
  
Chromatography ID:CH001780
Chromatography Summary:The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC.
Instrument Name:Agilent 1290 Infinity
Column Name:Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um)
Column Temperature:35
Flow Gradient:100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column.
Flow Rate:0.3 mL/min
Solvent A:95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Solvent B:5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Chromatography Type:HILIC
  
Chromatography ID:CH001781
Chromatography Summary:The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC.
Instrument Name:Agilent 1290 Infinity
Column Name:Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um)
Column Temperature:35
Flow Gradient:100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column.
Flow Rate:0.3 mL/min
Solvent A:95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Solvent B:5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate
Chromatography Type:HILIC
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