Return to study ST001450 main page
MB Sample ID: SA123954
Local Sample ID: | 114 |
Subject ID: | SU001524 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002421 | AN002422 | AN002423 | AN002424 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | HILIC | HILIC |
Chromatography system | Agilent 1260 Infinity | Agilent 1260 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | QTOF | QTOF |
MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF | Agilent 6545 QTOF | Agilent 6545 QTOF |
Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH001778 |
Chromatography Summary: | The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC. |
Instrument Name: | Agilent 1260 Infinity |
Column Name: | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) |
Column Temperature: | 35 |
Flow Gradient: | 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. |
Flow Rate: | 0.3 mL/min |
Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Solvent B: | 5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Chromatography Type: | HILIC |
Chromatography ID: | CH001779 |
Chromatography Summary: | The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC. |
Instrument Name: | Agilent 1260 Infinity |
Column Name: | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) |
Column Temperature: | 35 |
Flow Gradient: | 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. |
Flow Rate: | 0.3 mL/min |
Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Solvent B: | 5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Chromatography Type: | HILIC |
Chromatography ID: | CH001780 |
Chromatography Summary: | The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC. |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) |
Column Temperature: | 35 |
Flow Gradient: | 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. |
Flow Rate: | 0.3 mL/min |
Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Solvent B: | 5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Chromatography Type: | HILIC |
Chromatography ID: | CH001781 |
Chromatography Summary: | The mobile phase consisted of (A) H2O:ACN (95:5, v/v) with 5 mM ammonium acetate and 0.1% acetic acid, and (B) H2O:ACN (5:95, v/v), 5 mM ammonium acetate and 0.1% acetic acid. Gradient elution was performed as follows: 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. The flow rate was 0.3 mL/min, the injection volume was 5 µL, followed by an H2O:ACN (5:95, v/v) needle wash for 10 s, and the column temperature was 35 oC. |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters XBridge BEH Amide (15 cm x 2.1mm,2.5um) |
Column Temperature: | 35 |
Flow Gradient: | 100% mobile phase B for 1.5 min, 100 to 78% B from 1.5-6.0 min, 78 to 50% B from 6.0-9.0 min, 50% B from 9.0-15.0 min, restoration to 100% B from 15.0-17.0 min, and continued 100% B from 17.0-30.0 to equilibrate the LC column. |
Flow Rate: | 0.3 mL/min |
Solvent A: | 95% water/5% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Solvent B: | 5% water/95% acetonitrile; 0.1% acetic acid; 5 mM ammonium acetate |
Chromatography Type: | HILIC |