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MB Sample ID: SA125866

Local Sample ID:A1
Subject ID:SU001566
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Fischer 344
Age Or Age Range:6 wk
Gender:Female
Animal Animal Supplier:Envigo

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Combined analysis:

Analysis ID AN002474
Analysis type MS
Chromatography type HILIC
Chromatography system Eksigent microLC 200
Column Phenomenex Luna NH2(150 x 1.0mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5600+ TripleTOF
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH001812
Chromatography Summary:The mobile phases for separation consisted of water (A) and acetonitrile (B), both supplemented with 5 mM ammonium acetate and adjusted to pH 7.3 using ammonium hydroxide. The gradient proceeded at a flow rate of 30 μL/min as follows: 98% B at 0 min, 95% B at 1 min, 80% B at 5 min, 46% B at 6 min, 14.7% B at 13 min, 0% B at 17 min, 100% B at 17.1 min, and 100% B at 23 min.
Chromatography Comments:Phenomenex (Luna 3 μ NH2 100 Å, 150 mm × 1.0 mm)
Instrument Name:Eksigent microLC 200
Column Name:Phenomenex Luna NH2(150 x 1.0mm,3um)
Solvent A:100% water
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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