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MB Sample ID: SA154480
Local Sample ID: | GOLPH3_OE_2 |
Subject ID: | SU001751 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Combined analysis:
Analysis ID | AN002732 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Shimadzu Prominence UFPLC xr system |
Column | HILIC Kinetex (50 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
Ion Mode | POSITIVE |
Units | intensities |
Chromatography:
Chromatography ID: | CH002018 |
Chromatography Summary: | Lipid extracts (2 μL injection volume in ChCl3:MeOH 2:1) were separated over an 8 minutes gradient at a flow rate of 200 μL/min on a HILIC Kinetex Column (2.6lm, 2.1 × 50 mm2) on a Shimadzu Prominence UFPLC xr system (Tokyo, Japan). Mobile phase A was acetonitrile:methanol 10:1 (v/v) containing 10 mM ammonium formate and 0.5% formic acid while mobile phase B was deionized water containing 10 mM ammonium formate and 0.5% formic acid. The elution of the gradient began with 5% B at a 200 μL/min flow and increased linearly to 50% B over 7 min, then the elution continued at 50% B for 1.5 min and finally, the column was re-equilibrated for 2.5 min. |
Instrument Name: | Shimadzu Prominence UFPLC xr system |
Column Name: | HILIC Kinetex (50 x 2.1mm,2.6um) |
Flow Gradient: | The elution of the gradient began with 5% B and increased linearly to 50% B over 7 min, then the elution continued at 50% B for 1.5 min and finally, the column was re-equilibrated for 2.5 min. |
Flow Rate: | 200 µL/min |
Solvent A: | 91% acetonitrile/9% methanol; 0.5% formic acid; 10 mM ammonium formate |
Solvent B: | 1005 water; 0.5% formic acid; 10 mM ammonium formate |
Chromatography Type: | HILIC |