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MB Sample ID: SA154483
| Local Sample ID: | CTRL_2 |
| Subject ID: | SU001751 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002732 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu Prominence UFPLC xr system |
| Column | HILIC Kinetex (50 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Ion Mode | POSITIVE |
| Units | intensities |
Chromatography:
| Chromatography ID: | CH002018 |
| Chromatography Summary: | Lipid extracts (2 μL injection volume in ChCl3:MeOH 2:1) were separated over an 8 minutes gradient at a flow rate of 200 μL/min on a HILIC Kinetex Column (2.6lm, 2.1 × 50 mm2) on a Shimadzu Prominence UFPLC xr system (Tokyo, Japan). Mobile phase A was acetonitrile:methanol 10:1 (v/v) containing 10 mM ammonium formate and 0.5% formic acid while mobile phase B was deionized water containing 10 mM ammonium formate and 0.5% formic acid. The elution of the gradient began with 5% B at a 200 μL/min flow and increased linearly to 50% B over 7 min, then the elution continued at 50% B for 1.5 min and finally, the column was re-equilibrated for 2.5 min. |
| Instrument Name: | Shimadzu Prominence UFPLC xr system |
| Column Name: | HILIC Kinetex (50 x 2.1mm,2.6um) |
| Flow Gradient: | The elution of the gradient began with 5% B and increased linearly to 50% B over 7 min, then the elution continued at 50% B for 1.5 min and finally, the column was re-equilibrated for 2.5 min. |
| Flow Rate: | 200 µL/min |
| Solvent A: | 91% acetonitrile/9% methanol; 0.5% formic acid; 10 mM ammonium formate |
| Solvent B: | 1005 water; 0.5% formic acid; 10 mM ammonium formate |
| Chromatography Type: | HILIC |
