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MB Sample ID: SA154643
Local Sample ID: | C18-neg-HFD-Drug-217b |
Subject ID: | SU001757 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002738 | AN002739 | AN002740 | AN002741 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Normal phase | Normal phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
Column | Cogent Diamond Hydride (150 × 2.1mm,3.5um) | Cogent Diamond Hydride (150 × 2.1mm,3.5um) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | QTOF | QTOF |
MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | abundance | abundance | abundance | abundance |
Chromatography:
Chromatography ID: | CH002024 |
Chromatography Summary: | The mobile phase for ANP separation consisted of 6 mM EDTA and 0.025% acetic acid in isopropanol:H2O (50:50) (solvent A) and 6 mM EDTA and 5mM ammonium acetate in CH3CN: H2O (90:10) (solvent B). Gradient steps were applied as follows: 0–1 min, 99% B; 1–15 min, to 20% B; 15.1–29 min, 0% B; 29.01-35 min, 99% B. |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Cogent Diamond Hydride (150 × 2.1mm,3.5um) |
Chromatography Type: | Normal phase |
Chromatography ID: | CH002025 |
Chromatography Summary: | The mobile phase for RP separation consisted of 1 mM ammonium formate and 0.1% formic acid in H2O (solvent A) and 0.1% formic acid in CH3CN (solvent B). The gradient was as follows: 0–2 min, 1% B; 2– 20 min, to 50% B; 20–25 min, to 85% B; 25-30 min, to 99% B; 30.01-36 min, 1% B. |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
Flow Gradient: | 0-2 min, 1% B; 2- 20 min, to 50% B; 20-25 min, to 85% B; 25-30 min, to 99% B; 30.01-36 min, 1% B |
Solvent A: | 100% water; 0.1% formic acid; 1 mM ammonium formate |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |