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MB Sample ID: SA167193
Local Sample ID: | cele_HFD_1 |
Subject ID: | SU001873 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 6-7 weeks |
Gender: | Male |
Animal Housing: | temperature-controlled room under specific-pathogen free (SPF) or standard animal housing conditions with free access to food and water |
Animal Feed: | semi-purified normal chow diet (control fat diet (CFD), 7% w/w fat and 16% total energy from fat; AIN93G, Specialty Feeds Pty Ltd, Australia) or high fat diet (HFD, 36% w/w fat and 59% total energy from fat; SF03-002, Specialty Feeds Pty Ltd, Australia) |
Animal Water: | ad libitum |
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Combined analysis:
Analysis ID | AN002917 | AN002918 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) | Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | height | height |
Chromatography:
Chromatography ID: | CH002160 |
Chromatography Summary: | Lipidomics analysis was performed using reversed phase liquid chromatography and high-resolution mass spectrometry. Samples (10 µl) were injected onto a Dionex Ultimate 3000 UHPLC system (Thermo Scientific, Australia) fitted with an analytical C8 column (100 x 2.1 mm; 2.7 µm, Sigma Aldrich, Australia). Chromatography was performed using solvent A (2 mM formic acid, 8 mM ammonium formate, 40% v/v isopropanol) and solvent B (2 mM formic acid, 8 mM ammonium formate, 98% v/v isopropanol) as mobile phases with a 30 min gradient starting at 0% B and increasing to 35% B from 0 to 8 min, then to 50% B from 8-16 min, then to 80% B from 16-19 min, then finally to 100% B by 23 min. 100% B was maintained for a further 3 min before equilibrating to 0% by 28 min and washing for a further 2 min. |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Sigma Aldrich,Supleco,C8 (100 x 2.1mm,2.7um) |
Column Temperature: | 40C |
Flow Gradient: | 0 min - 0%B, 8 min - 35%B, 16 min - 50%B, 19 min - 80%B, 23 min 100%B, 26 min - 100%B, 28 min - 0%B |
Flow Rate: | 0.2 ml/min |
Injection Temperature: | 4 |
Solvent A: | 40% isopropanol/60% water; 2 mM formic acid; 8 mM ammonium formate, |
Solvent B: | 98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium formate |
Analytical Time: | 30 min |
Oven Temperature: | 40C |
Sample Loop Size: | 25 uL |
Sample Syringe Size: | 25 uL |
Chromatography Type: | Reversed phase |