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MB Sample ID: SA168909
| Local Sample ID: | C33A |
| Subject ID: | SU001891 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002940 | AN002941 | AN002942 | AN002943 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | GC | Normal phase |
| Chromatography system | Agilent 1200 | Agilent 1200 | Agilent 7890A | Agilent 7100 CE |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) | Agilent DB5-MS (30m x 0.25mm, 0.25um) | Agilent Technologies fused silica capillary 96cm x 50μm |
| MS Type | ESI | ESI | EI | ESI |
| MS instrument type | QTOF | QTOF | Single quadrupole | TOF |
| MS instrument name | Agilent 6520 QTOF | Agilent 6520 QTOF | Agilent 5975C | Agilent 6224 TOF |
| Ion Mode | POSITIVE | NEGATIVE | UNSPECIFIED | POSITIVE |
| Units | AREA | AREA | AREA |
Chromatography:
| Chromatography ID: | CH002177 |
| Chromatography Summary: | The analysis of the samples was accomplished using an UHPLC system (1200 Infinity system, Agilent Technologies, Waldbronn, Germany), coupled to a 6520 QTOF MS (Agilent Technologies) with an ESI ion source. The sample injection volume was set up to 10 μL. The separation was achieved using a Discovery® HS C18 15cm x 2.1 mm, 3 µm (Supelco analytical) reverse phase column at thermostated 40 °C. |
| Instrument Name: | Agilent 1200 |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,2.6um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002178 |
| Chromatography Summary: | An Agilent GC system (7890A) coupled to a- 5975C mass spectrometer (Agilent Technologies) was used to perform metabolite fingerprinting of plasma samples. Briefly, 2 μL of derivatized samples were automatically injected in split mode (ratio 1:10) through a split liner of ultra-inert deactivated glass wool from Agilent. The separation of the compounds was achieved using a pre-column (10 m J&W integrated with Agilent 122‐5532G) combined with a GC DB5-MS column (length, 30 m; internal diameter, 0.25 mm; and 0.25 μm film of 95% of dimethyl/5% diphenylpolysiloxane). |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
| Chromatography ID: | CH002179 |
| Chromatography Summary: | The analysis was performed using a 7100 capillary electrophoresis (Agilent Technologies) coupled to a TOF MS 6224 mass spectrometer (Agilent Technologies), equipped with an ESI ion source. For the separation of metabolites, an Agilent Technologies fused silica capillary (total length, 96 cm; internal diameter, 50 μm) was used, working in normal polarity. Before each analysis, the capillary was washed for 5 min (950 mbar) with background electrolyte (BGE) (0.8 M formic acid solution in 10% methanol (v/v)). The sample injection was performed during 50 s at 50 mbar and, in order to improve the reproducibility of the analysis, the BGE was injected for 20 s at 100 mbar after the injection of each sample. |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Agilent Technologies fused silica capillary 96cm x 50μm |
| Chromatography Type: | Normal phase |
