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MB Sample ID: SA172899
| Local Sample ID: | KWS10653 |
| Subject ID: | SU001928 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002999 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Exion LC |
| Column | InertSustain C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex Triple Quad 6500+ |
| Ion Mode | UNSPECIFIED |
| Units | nM |
Chromatography:
| Chromatography ID: | CH002224 |
| Chromatography Summary: | 2 μL was injected to LC/ESI-MS/MS (Triple Quad 6500+ tandem mass spectrometer, equipped with an ESI probe and Exion LC AD ultra-high pressure liquid chromatography system; SCIEX). A separation column, InertSustain C18 (150 mm × 2.1 mm ID, 3 μm particle size; GL Sciences Inc.), was utilized at 40 °C. A mixture of 10 mM ammonium acetate and acetonitrile was used as the eluent and the separation was carried out by linear gradient elution at a flow rate of 0.2 mL/min. The mobile phase composition was gradually changed as follows: ammonium acetate-acetonitrile (86:14, v/v) for 0.5 min, (78:22, v/v) for 0.5-5 min, (72:28, v/v) for 5-28 min, (46:54, v/v) for 28-55 min, (2:98, v/v) for 55-66 min, and (2:98, v/v) for 4 min. The total run time was 70 min. |
| Instrument Name: | Exion LC |
| Column Name: | InertSustain C18 |
| Column Temperature: | 40 |
| Flow Gradient: | ammonium acetate-acetonitrile (86:14, v/v) for 0.5 min, (78:22, v/v) for 0.5-5 min, (72:28, v/v) for 5-28 min, (46:54, v/v) for 28-55 min, (2:98, v/v) for 55-66 min, and (2:98, v/v) for 4 min. The total run time was 70 min. |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 100% water; 10mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
