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MB Sample ID: SA176889
Local Sample ID: | Ding_A-5_005 |
Subject ID: | SU001987 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | ATF3(+/+) vs ATF3(-/-) |
Cell Strain Details: | HCT116 cells engineered to knockout ATF3 expression via rAAV-mediated homologous recombination |
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Combined analysis:
Analysis ID | AN003106 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Agilent 7890A |
Ion Mode | UNSPECIFIED |
Units | enrichment percentage |
Chromatography:
Chromatography ID: | CH002293 |
Chromatography Summary: | Gas chromatography-Quadrupole-time of flight mass spectrometry (GC-Q-TOF MS) was used for Stable Isotope enrichment. Rtx-5Sil MS column (30m length, 0.25 mm i.d, 0.25 μM 95% dimethyl 5% diphenyl polysiloxane film) with an additional 10 m guard column was installed on Agilent 7890 GC (Agilent Technologies). 99.9999% pure Helium gas was used as a mobile phase with a flow rate of 1mL/min. GC temperature was held at 50°C for 1 min, ramped at 20°C/min to 330°C and then held for 5 min. Electron ionization at -70eV was employed on a Agilent 7250 Quadrupole time of flight mass spectrometer (Agilent Technologies) with ion source temperature at 250°C and detector voltage at 1850V. Mass spectra were acquired at an acquisition rate of 5 spectra/s, with a scan range of 85-900 Da. |
Instrument Name: | Agilent 7890A |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |