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MB Sample ID: SA177866
| Local Sample ID: | 0060_LC_20190903_sample_0001 |
| Subject ID: | SU001997 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003118 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6545 LC/QTOF |
| Column | Waters ACQUITY UPLC BEH C18 |
| MS Type | ESI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 6545 LC/QTOF |
| Ion Mode | UNSPECIFIED |
| Units | Summarised value |
Chromatography:
| Chromatography ID: | CH002303 |
| Chromatography Summary: | The extracts were analyzed on a Waters Q-Tof Premier mass spectrometer combined with an Acquity Ultra Performance LCTM. The column (at 50 °C) was an Acquity UPLCTM BEH C18 2.1 × 100 mm with 1.7 µm particles. The solvent system included A. ultrapure water (1% 1 M NH4Ac, 0.1% HCOOH) and B. LC/MS grade acetonitrile/isopropanol (1:1, 1% 1M NH4Ac, 0.1% HCOOH). The gradient started from 65% A / 35% B, reached 80% B in 2 min, 100% B in 7 min and remained there for 7 min. The flow rate was 0.400 ml/min and the injected amount was 2.0 µL (Acquity Sample Organizer, at 10 °C). Reserpine was used as the lock spray reference compound. The lipid profiling was carried out using electrospray ionization mode and the data were collected at a mass range of m/z 300-1200 with a scan duration of 0.2 sec. |
| Instrument Name: | Agilent 6545 LC/QTOF |
| Column Name: | Waters ACQUITY UPLC BEH C18 |
| Column Temperature: | 50 |
| Flow Gradient: | The gradient started from 65% A / 35% B, reached 80% B in 2 min, 100% B in 7 min and remained there for 7 min. |
| Flow Rate: | 0.400 ml/min |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol 0.1% formic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
