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MB Sample ID: SA181782
| Local Sample ID: | N23 |
| Subject ID: | SU002011 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN003143 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | The Shim-pack UFLC SHIMADZU CBM A ultrahigh-performance liquid chromatography (UHPLC) system |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Ion trap |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | µM |
Chromatography:
| Chromatography ID: | CH002325 |
| Chromatography Summary: | The Shim-pack UFLC SHIMADZU CBM A ultrahigh-performance liquid chromatography (UHPLC) system (SHIMADZU, Japan) coupled with QTRAP 6500+ triple quadrupole mass spectrometer (Sciex, Washington, USA) was used to analyze the metabolomics. ACQUITY UPLC BEH C18 1.7 µm VanGuard pre-column (2.1mm × 5 mm) and ACQUITY UPLC BEH C18 1.7 µm analytical column (2.1 × 100 mm) (Waters Corporation, Milford, MA, USA) were applied to this system. The mobile phases A and B were 0.1% formic acid solution in water and acetonitrile-IPA mixture (70:30, v/v), respectively. A 5-μL injection of each sample was maintained at 40 °C and the flow rate was 0.40 mL/min. The mobile phase gradient was: 0-1 min (5% B), 1-11min (5-78% B), 11-13.5 min (78-95% B), 13.5-14 min (95-100% B), 14-16 min (100% B), 16-16.1 min (100-5% B), and 16.1-18 min (5% B). |
| Instrument Name: | The Shim-pack UFLC SHIMADZU CBM A ultrahigh-performance liquid chromatography (UHPLC) system |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-1 min (5% B), 1-11min (5-78% B), 11-13.5 min (78-95% B), 13.5-14 min (95-100% B), 14-16 min (100% B), 16-16.1 min (100-5% B), and 16.1-18 min (5% B). |
| Flow Rate: | 0.40 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 70% acetonitrile/30% isopropanol |
| Chromatography Type: | Reversed phase |
