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MB Sample ID: SA184001
| Local Sample ID: | 25A_T1_I1_G2_L1 |
| Subject ID: | SU002028 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley |
| Age Or Age Range: | 8 weeks |
| Weight Or Weight Range: | 250-450 g |
| Gender: | Male and female |
| Animal Animal Supplier: | Charles River |
| Animal Light Cycle: | 12 h reverse light-dark cycles |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003174 | AN003175 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C30 (50 x 2.1mm,2.1um) | Thermo Accucore C30 (50 x 2.1mm,2.1um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo ID-X Orbitrap Tribrid | Thermo ID-X Orbitrap Tribrid |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized Peak Area | Normalized Peak Area |
Chromatography:
| Chromatography ID: | CH002347 |
| Chromatography Summary: | All samples were run in a randomized order over two and a half days of consecutive instrument time. QC samples were interleaved every 24 runs to evaluate LC-MS system stability and to account for time-dependent batch effects. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore C30 (50 x 2.1mm,2.1um) |
| Column Temperature: | 60 ℃ |
| Flow Gradient: | (Time: A/B) 0 min 80/20, 1 min 40/60, 5 min 30/70, 5.5 min 15/85, 8 min 10/90, 8.2 min 0/100, 10.7 min 80/20, 12 min 80/20 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
