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MB Sample ID: SA184053
| Local Sample ID: | 2 |
| Subject ID: | SU002030 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003177 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | Dionex UltiMate 3000 RSLC |
| Column | HILICpak VT50 2D (150 mm x 2.0 mm,5um particle size,Shodex,Japan) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH002349 |
| Chromatography Summary: | Liquid chromatography separation was achieved on a HILICpak VT50 2D column (150 mm x 2.0 mm, 5 µm particle size, Shodex, Japan). Buffer A consists of 90% acetonitrile, 10% water, containing 20 mM Triethylamine : Formic acid at pH 9.18; Buffer B consists of 5% acetonitrile, 95% water containing 54 mM Triethyl-amine : Formic acid at pH 3.03. Flow rate is 0.2mL/min from 0 to 5 minutes, then 0.3 mL/min from 5.1 to 58 min, and reduced again to 0.2mL/min from 58.1 to 60 min. The gradient starts with 0%B from 0 to 10 min, then increases linearly from 0 to 16%B from 10 to 27 min, up to 65%B at 32 min, 87%B at 34 min, 100%B hold from 34.1 to 47 min, then 0%B from 47.1 to 60 min. |
| Instrument Name: | Dionex UltiMate 3000 RSLC |
| Column Name: | HILICpak VT50 2D (150 mm x 2.0 mm,5um particle size,Shodex,Japan) |
| Chromatography Type: | Unspecified |
