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MB Sample ID: SA185576
Local Sample ID: | Pooled Human Plasma 5 |
Subject ID: | SU002061 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Pooled |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002856 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 Infinity II |
Column | Waters Acquity BEH Amide (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6545 |
Ion Mode | POSITIVE |
Units | Total Intensity Units |
Chromatography:
Chromatography ID: | CH002383 |
Chromatography Summary: | HILIC separations were performed on a Waters Acquity UPLC BEH amide column (2.1 x 100 mm 1.7 m) with a matching Vanguard precolumn. The flow rate was set to 0.3 mL/min and mobile phases consisted of (A) 95:5 acetonitrile/water with 0.125% v/v formic acid and 10 mM ammonium formate and (B) 95:5 water/acetonitrile with 0.125% v/v formic acid and 10 mM ammonium formate. Gradient specified in protocol file. |
Methods Filename: | 2 mm amide pos MS2 70%H2O 120min_PC6.m |
Instrument Name: | Agilent 1290 Infinity II |
Column Name: | Waters Acquity BEH Amide (100 x 2.1mm,1.7um) |
Column Pressure: | 300 bar |
Column Temperature: | 55 |
Flow Rate: | 0.3 mL/min |
Sampling Cone: | 175 V |
Solvent A: | 95% acetonitrile/5% water; 0.125% formic acid; 10 mM ammonium formate |
Solvent B: | 5% acetonitrile/95% water; 0.125% formic acid; 10 mM ammonium formate |
Analytical Time: | 120 min |
Capillary Voltage: | 3500 V |
Weak Wash Solvent Name: | 85:15 acetonitrile:water |
Target Sample Temperature: | 4 |
Sample Loop Size: | 20 uL |
Chromatography Type: | HILIC |