Return to study ST002056 main page
MB Sample ID: SA193793
| Local Sample ID: | CarnsTG2 |
| Subject ID: | SU002138 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Age Or Age Range: | 8-12 weeks |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003348 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Agilent DB5-MS (60m × 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | UNSPECIFIED |
| Units | intensity |
Chromatography:
| Chromatography ID: | CH002479 |
| Chromatography Summary: | The extracted and derivatized samples were analyzed on a LECO Pegasus GC×GC-TOF MS instrument (LECO Corp., St. Joseph, MI, USA) coupled to an Agilent 6890 gas chromatography and a Gerstel MPS2 autosampler (GERSTEL Inc., Linthicum, MD, USA), featuring a LECO two-stage cryogenic modulator and secondary oven. The primary column was a 60 m × 0.25 mm 1dc × 0.25 µm 1df DB-5 ms GC capillary column (phenyl arylene polymer virtually equivalent to (5%-phenyl)-methylpolysiloxane). The secondary GC column 1 m × 0.25 mm 2dc × 0.25 µm 2df, DB-17 ms ((50% phenyl)-methylpolysiloxane) was placed inside the secondary GC oven following the thermal modulator. Both columns were obtained from Agilent Technologies (Agilent Technologies J&W, Santa Clara, CA, USA) and were connected through a press fit connector. The helium carrier gas (99.999% purity) flow rate was set to 2.0 mL/min at a corrected constant flow via pressure ramps. The inlet temperature was set at 280 °C. The primary column temperature was programmed with an initial temperature of 60 °C for 0.5 min, then ramped at 5°C/min to 270 °C, and maintained for 15 min. The secondary column temperature program was set to an initial temperature of 70 °C for 0.5 min and then ramped at the same temperature gradient employed in the first column to 280 °C, accordingly. The thermal modulator was set to 15 °C relative to the primary oven and a modulation time was 2s. The mass range was set as 29-800 m/z with an acquisition rate of 200 mass spectra per second. The ion source chamber was 230°C with the transfer line temperature of 280°C, and the detector voltage was 1440 V with electron energy of 70 eV. The acceleration voltage was turned on after a solvent delay of 544 s, the split ratio was set at 10:1. |
| Instrument Name: | Agilent 6890N |
| Column Name: | Agilent DB5-MS (60m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
