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MB Sample ID: SA194026
| Local Sample ID: | WT3M2 |
| Subject ID: | SU002143 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003360 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | ThermoFisher Ultimate 3000 UHPLC system |
| Column | Waters BEH Amide |
| MS Type | ESI |
| MS instrument type | Hybrid Quadrupole-Orbitrap™ |
| MS instrument name | ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) |
| Ion Mode | NEGATIVE |
| Units | pmoles/I |
Chromatography:
| Chromatography ID: | CH002486 |
| Chromatography Summary: | Chromatographic separation was performed on a ThermoFisher Ultimate 3000 UHPLC system with a Waters BEH Amide column (2.1mm × 100 mm, 1.7 μm). The injection volume was 2μL and the flow rate was 0.25 mL/min. The column temperature was 15°C. The mobile phases consisted of water with 0.01% formic acid and 2 mM ammonium formate (phase A) and acetonitrile (phase B). A linear gradient elution was performed with the following program: 0 min, 90%B; 4 min, 85% B; 11 min, 75%B; 14 min, 70%B, 14.5min, 50%B and held to 17 min; 17.1 min, 90%B and held to 20.01 min. |
| Instrument Name: | ThermoFisher Ultimate 3000 UHPLC system |
| Column Name: | Waters BEH Amide |
| Column Temperature: | 15 |
| Flow Gradient: | 0 min, 90%B; 4 min, 85% B; 11 min, 75%B; 14 min, 70%B, 14.5min, 50%B and held to 17 min; 17.1 min, 90%B and held to 20.01 min. |
| Flow Rate: | 0.25ml/min |
| Solvent A: | 100% water; 0.01% formic acid; 2 mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
