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MB Sample ID: SA194737
Local Sample ID: | 25 P1M |
Subject ID: | SU002152 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male |
Cell Strain Details: | HCT 116 |
Subject Comments: | HCT 116 cell line was cultured as a 2D monolayer and 3D spheroids |
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Combined analysis:
Analysis ID | AN003374 | AN003375 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1260 | Agilent 1260 |
Column | Thermo Accucore C30 (100 x 2.1mm,2.6um) | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | pmol lipid/ ug protein | pmol lipid/ ug protein |
Chromatography:
Chromatography ID: | CH002494 |
Instrument Name: | Agilent 1260 |
Column Name: | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
Column Temperature: | 50 |
Flow Rate: | 400uL/min |
Injection Temperature: | 4 |
Internal Standard: | equiSPLASH LIPIDOMIX |
Sample Injection: | 2 |
Solvent A: | 50% water/50% acetonitrile; 10mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
Chromatography Type: | Reversed phase |