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MB Sample ID: SA194806
| Local Sample ID: | SS-007 |
| Subject ID: | SU002155 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | 3rd instar larvae |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003378 | AN003379 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | UltiMate 3000RSLC HPLC (Thermo Scientific) | UltiMate 3000RSLC HPLC (Thermo Scientific) |
| Column | Phenomenex Luna NH2 (150 x 2mm) | Phenomenex Luna NH2 (150 x 2mm) |
| MS Type | ESI | ESI |
| MS instrument type | Ion trap | Ion trap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity (area under the curve) | intensity (area under the curve) |
Chromatography:
| Chromatography ID: | CH002497 |
| Chromatography Summary: | Separation was achieved using a linear gradient from 15% to 90% A over 18 min. This was followed by an isocratic step at 90% A for 9 min and re-equilibration to the initial 15% A for 7 min. |
| Instrument Name: | UltiMate 3000RSLC HPLC (Thermo Scientific) |
| Column Name: | Phenomenex Luna NH2 (150 x 2mm) |
| Column Temperature: | 27 C |
| Flow Rate: | 200 ul/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
