Return to study ST002073 main page
MB Sample ID: SA194848
| Local Sample ID: | BileAcid_HI_022421_1936 |
| Subject ID: | SU002156 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003380 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo TSQ Vantage |
| Ion Mode | NEGATIVE |
| Units | ng/mL |
Chromatography:
| Chromatography ID: | CH002498 |
| Chromatography Summary: | Samples were analyzed using a Thermofisher Vanquish UHPLC system coupled to a Thermofisher TSQ Altis triple-quadrupole mass spectrometer. An Aquity BEH C18 column (1.7 µm, 2.1 mm×100 mm) with guard column Acquity BEH C18 (1.7 µm, 2.1 mm×5 mm) was used for chromatographic separation with mobile phases of A: LC-MS-grade water with 0.1% formic acid, and B: LC-MS-grade acetonitrile with 0.1% formic acid with a flow rate of 400 µL/min and column temperature of 50 °C. The gradient began at 20% B for 1 min, then shifted to 45% B between 1 and 11 min, then to 95% B between 11 and 14 min, then to 99% B between 14 and 14.5 min, 99% B was maintained until 15.5 min, then transitioned from 99% B to 20% B between 15.5 and 16.5 min, and maintained at 20% B until 18 min. Injection volume was 5 µL and MRM scans were collected for all bile acids and internal standards. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | The gradient began at 20% B for 1 min, then shifted to 45% B between 1 and 11 min, then to 95% B between 11 and 14 min, then to 99% B between 14 and 14.5 min, 99% B was maintained until 15.5 min, then transitioned from 99% B to 20% B between 15.5 and 16.5 min, and maintained at 20% B until 18 min. |
| Flow Rate: | 400 uL/min |
| Sample Injection: | 5 ul |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
