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MB Sample ID: SA198209
| Local Sample ID: | D0D1_A-63_2018-03-28 |
| Subject ID: | SU002166 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 47-89 years |
| Gender: | Male and female |
| Human Inclusion Criteria: | Patients with lung cancer |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003396 | AN003397 | AN003398 | AN003399 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Agilent 6550 | Agilent 6550 | Agilent 6550 | Agilent 6550 |
| Column | Waters Atlantis dC18 (100 x 3mm,3um) | Waters Atlantis dC18 (100 x 3mm,3um) | Waters BEH Amide (150 x 3.0mm,1.7um) | Waters BEH Amide (150 x 3.0mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
| Units | Values are raw peak area | raw peak area | raw peak area | Values are raw peak area |
Chromatography:
| Chromatography ID: | CH002511 |
| Chromatography Summary: | LC method 1: employed an Atlantis dC18 column (3x100 mm, 3 µm, Waters, UK) maintained at 60 °C with flow rate at 0.4 mL/min. Mobile phases were (A) water and (B) methanol both containing 5 mmol/L ammonium formate and 0.1 % formic acid. The elution gradient started at 5 % B at 0 to 1 min increasing linearly to 100 % by 12 min, held at 100 % B until 14 min, returning to 95 % A for 5 min. |
| Instrument Name: | Agilent 6550 |
| Column Name: | Waters Atlantis dC18 (100 x 3mm,3um) |
| Column Temperature: | 60 |
| Flow Gradient: | The elution gradient started at 5 % B at 0 to 1 min increasing linearly to 100 % by 12 min, held at 100 % B until 14 min, returning to 95 % A for 5 min |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1 % formic acid; 5 mM ammonium formate |
| Solvent B: | 100% methanol; 0.1 % formic acid; 5 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002512 |
| Chromatography Summary: | LC method 2: used a BEH amide column (3x150 mm, 1.7 µm, Waters, UK) maintained at 40 °C with flow rate at 0.6 mL/min. Mobile phases were (A) water and (B) acetonitrile both containing 0.1 % formic acid. The elution gradient started at 99 % B, decreasing linearly to 30 % from 1 to 12 min, held at 30 % B until 12.6 min, returning to 99 % B for 3.4 min. Sample injection volume was 1 µL for both LC methods. |
| Instrument Name: | Agilent 6550 |
| Column Name: | Waters BEH Amide (150 x 3.0mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | The elution gradient started at 99% B, decreasing linearly to 30% from 1 to 12 min, held at 30% B until 12.6 min, returning to 99% B for 3.4 min. |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
