Return to study ST002082 main page

MB Sample ID: SA198209

Local Sample ID:D0D1_A-63_2018-03-28
Subject ID:SU002166
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:47-89 years
Gender:Male and female
Human Inclusion Criteria:Patients with lung cancer

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN003396 AN003397 AN003398 AN003399
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Agilent 6550 Agilent 6550 Agilent 6550 Agilent 6550
Column Waters Atlantis dC18 (100 x 3mm,3um) Waters Atlantis dC18 (100 x 3mm,3um) Waters BEH Amide (150 x 3.0mm,1.7um) Waters BEH Amide (150 x 3.0mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6550 QTOF Agilent 6550 QTOF Agilent 6550 QTOF Agilent 6550 QTOF
Ion Mode NEGATIVE POSITIVE NEGATIVE POSITIVE
Units Values are raw peak area raw peak area raw peak area Values are raw peak area

Chromatography:

Chromatography ID:CH002511
Chromatography Summary:LC method 1: employed an Atlantis dC18 column (3x100 mm, 3 µm, Waters, UK) maintained at 60 °C with flow rate at 0.4 mL/min. Mobile phases were (A) water and (B) methanol both containing 5 mmol/L ammonium formate and 0.1 % formic acid. The elution gradient started at 5 % B at 0 to 1 min increasing linearly to 100 % by 12 min, held at 100 % B until 14 min, returning to 95 % A for 5 min.
Instrument Name:Agilent 6550
Column Name:Waters Atlantis dC18 (100 x 3mm,3um)
Column Temperature:60
Flow Gradient:The elution gradient started at 5 % B at 0 to 1 min increasing linearly to 100 % by 12 min, held at 100 % B until 14 min, returning to 95 % A for 5 min
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1 % formic acid; 5 mM ammonium formate
Solvent B:100% methanol; 0.1 % formic acid; 5 mM ammonium formate
Chromatography Type:Reversed phase
  
Chromatography ID:CH002512
Chromatography Summary:LC method 2: used a BEH amide column (3x150 mm, 1.7 µm, Waters, UK) maintained at 40 °C with flow rate at 0.6 mL/min. Mobile phases were (A) water and (B) acetonitrile both containing 0.1 % formic acid. The elution gradient started at 99 % B, decreasing linearly to 30 % from 1 to 12 min, held at 30 % B until 12.6 min, returning to 99 % B for 3.4 min. Sample injection volume was 1 µL for both LC methods.
Instrument Name:Agilent 6550
Column Name:Waters BEH Amide (150 x 3.0mm,1.7um)
Column Temperature:40
Flow Gradient:The elution gradient started at 99% B, decreasing linearly to 30% from 1 to 12 min, held at 30% B until 12.6 min, returning to 99% B for 3.4 min.
Flow Rate:0.6 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B: 100% acetonitrile; 0.1% formic acid
Chromatography Type:HILIC
  logo