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MB Sample ID: SA201394
| Local Sample ID: | CH-1 |
| Subject ID: | SU002186 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003434 | AN003435 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | SCIEX ExionLC AD | SCIEX ExionLC AD |
| Column | Phenomenex Kinetex C18(100 x 3.0mm,2.6m) | Phenomenex Kinetex C18(100 x 3.0mm,2.6m) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH002538 |
| Chromatography Summary: | The resuspended samples were placed on the sample-tray at 4 ℃ with 1 μL-volume injection for targeted-lipidomics analysis, which was performed on an UHPLC Q-Trap/MS system (Qtrap 6500, SCIEX, USA). A Kinetex C18 column (100 mm × 3.0 mm, 2.6 μm; Phenomenex); mobile phase A was methanol: ACN: water = 1:1:1 (v/v/v) with 5 mM ammonium acetate, while mobile phase B was IPA with 5 mM ammonium acetate. The following gradient-elution program was 0-0.5 min, 80% A; 0.5-1.5 min, 80%-60% A; 1.5-3 min, 60%-40% A; 3-13 min, 40%-2% A; 13-13.1 min, 2%-80% A; 13.1-17 min, 80% A. The flow rate was at 0.3 mL/min and the column temperature was maintained at 50 ℃. |
| Instrument Name: | SCIEX ExionLC AD |
| Column Name: | Phenomenex Kinetex C18(100 x 3.0mm,2.6m) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-0.5 min, 80% A; 0.5-1.5 min, 80%-60% A; 1.5-3 min, 60%-40% A; 3-13 min, 40%-2% A; 13-13.1 min, 2%-80% A; 13.1-17 min, 80% A |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 33.3% methanol/33.3% acetonitrile/33.3% water; 5 mM ammonium acetate, 100% isopropanol; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
